The reversal activity of AnXa for apixaban and rivaroxaban was measured by a modified anti-FXa assay in a 96-well format previously used in the AnXa preclinical and clinical studies. The same set of contrived samples was assessed using the apixaban and rivaroxaban assays on the ACL TOP 700 ...
(working solution (2×): 1361 ng/mL or 20 nM in assay buffer, prepare prior to use). Chromogenic Substrates (Pharmacia Hepar Inc.) 1. S2222 (FXa assay) stock solution: 6 mM in deionized H2 O, store at 4° C. (working solution (4×): 656 μM in assay buffer). 2. S2302...
Subsequently, thereduction of factors X and II contributes to prolongationof the PT. The PT assay is performed by adding calciumand thromboplastin to citrated plasma. The responsiveness of a thromboplastin can bemeasured by assessing its international sensitivityindex (ISI). Highly sensitive ...
由于 LMWH 和普通肝素抗 凝作用主要抑制 FXa 的活性,利用发色底物法测定 LMWH 及 UFH 对 FXa 活性的抑制作 用已公认为检测抗凝程度的金标准[7] 。在血液透析中现在主要是低分子肝素和普通肝 素抗凝,但在长期血液透析中那种抗凝剂效果可,副作用少,同时那个范围抗 Xa 因 子活性水平可达到最佳的治疗水平。因此...
The final step was also carried out in the presence of radioactive PAP35S to prepare the radiolabeled compound represented by the structure of Formula I, in order to test its ability to bind to anti-thrombin III (ATIII) by gel mobility shift assay (22). The synthesized compound represented...
34 sara from patients with suspected HAT ware assessed with platelets of 4 donors using the aggregometer test (AT), the sero- tonin release assay (SRA) and the HIP-test concomitant- ly. HIP-test proved to be as sensitive and specific as SRA, which is time consuming and requires radio...
of FXa that formed was measured by addition of the chromogenic substrate Spectrozyme FXa in a Microplate Reader by determining the change in absorption at 405 nm and the parameters for the Michaelis-Menten kinetics were analysed by the method described by Ruf. The results show that both tTF and...
Annu Rev Entomol 52:209–229 Niedrig M, Kürsteiner O, Herzog C, Sonnenberg K (2008) Evaluation of an indirect immunofluorescence assay for detection of immuno- globulin M (IgM) and IgG antibodies against yellow fever virus. Clin Vaccine Immunol 15(2):177–181 Tomori O (2004) Yellow fever...
Annu Rev Entomol 52:209–229 Niedrig M, Kürsteiner O, Herzog C, Sonnenberg K (2008) Evaluation of an indirect immunofluorescence assay for detection of immuno- globulin M (IgM) and IgG antibodies against yellow fever virus. Clin Vaccine Immunol 15(2):177–181 Tomori O (2004) Yellow fever...
T24/83 cells (1 × 104 cells/well) seeded in a 96-well plate were treated with increasing doses of exogenous recombinant human GRP78 (hrGRP78) for 3 h, and TF PCA was measured using the continuous assay (n = 8). C, effect of exogenous recombinant human GRP78 (hrGRP78) on TF ...