METHODS: We separated and purified CD4 + T cells from mice, activated them in vitro, and co-cultured them with TLR ligands, MP or inhibitors of nuclear factor-kappa B (NF-κB) and activator protein 1 (AP-1). We then assessed CD4 + T cell survival and proliferation and the expression ...
We investigated T cell receptor induced IL-18 secretion, the cellular and molecular mechanisms associated with the induction of IL-18 and the role of IL-18 in IFN-γ production in the different stages of multiple sclerosis (MS). We found that anti-CD3/CD28 induced IL-18 production by periph...
Two hours after a Vpr-βlam infection assay, the primary non-activated HIV+ CD4+ T cells (blue) from an elite controller (EC) were co-cultured for 30 min with an autologous Gag-specific CD8+ T cell line (green), fixed, and stained for actin with phalloidin (orange) and perforin (red...
differentiation by up-regulating mitochondrial fatty acid oxidation (FAO) in activated CD4+T cells via AMP-activated protein kinase (AMPK) activation and mitochondrial membrane potential reduction. In addition, the AMPK agonist facilitated β1-AA-mediated FAO and nTreg cell differentiation. To further ...
CD4+T cells, albeitMat2amRNA stability and translation was unaltered (Fig.3a).Mthfd2is implicated in cell proliferation and enhanced flux through the mitochondrial branch of the folate cycle70,71,72and has been identified as an important metabolic checkpoint in CD4+T cells73. It showed ...
Following activation, cell surface CD154 is increased on CD4 T cells from pediatric lupus patients as compared with controls, and this increase correlates with the presence of nephritis, increased CD154 transcription rates, and increased NF-AT activity. These results suggest that NF-AT/calcineurin ...
cell adjuvant, novel strategies that can activate endogenous antitumor activity in cancer patients remain an unmet need. In this study, we established a new method to destroy pre-developed tumors and confer potent antitumor immunity in mice using alloantigen-activated CD4+(named AAA-CD4+) T cells...
Here, using magnetic beads and fluorescence-activated cell sorting, we extensively phenotype the Tck effector cells and conclude that effector function resides within the CD4+CD45RO+, CCR7-, CD49dhigh population, and that these cells are derived from the effector memory CD4+ T cells in resting...
Cloned αβ TcR + CD3 + CD4 CD8 T cells, with natural suppressor (NS) activity, were cocultured with thymocytes in the presence or absence of mitoge... PV Vlasselaer,M Fischer,S Strober,... - 《Cellular Immunology》 被引量: 3发表: 1991年 Lymphokine-activated killer cell activity of...
Lymphokine activated killer cell activity of CD4 − CD8 − TCR αβ + ... ARASE,H - 《J Immunol》 被引量: 55发表: 1993年 Generation of lymphokine-activated killer activity in T cells. Possible regulatory circuits. CD4+ and CD8+ T cells do not develop significant lymphokine-...