Primers for the Target Genes and β-actin Used in RT-PCR.Dehong WuLi XuChengping WenGuanqun XieJinjun JiJieli PanYifeng JiaoYongsheng Fan
Primers of phosphate-starvation-inducible (PSI) genes used for RT-PCR analysis Gene OsPHR1 OsPHR2 OsIPS1 OsIPS2 SQD2 OsPAP10 OsActin Primer sequence (5’-3’) F:CACAAGAAGGGAAAACTACCGATG R:TCAAGATTCATGCACTCTACGACGC F:CGCTTTGTAGATGCTGTCAATC R:AGACCCTCATCACATCCTCATTATC F:AAGGGCAGGG...
序列分析结果表明,建兰 Actin 基因长度为 1 434 bp,编码区长度为 1 134 bp,编码 377 个氨基酸,将其命名为 CeActin,GenBank 登录号为 JN613147.CeActin 推导的氨基酸序列与其他植物的同源性都较高,具有高 度的保守性.采用半定量 RT-PCR 技术分析 CeActin 在建兰各组织及花不同发育时期的表达情况,结果表明...
28S 18S 5S 图 1 海州香薷根部总 RNA 提取 2.2 RT-PCR扩增和克隆 以第一链 cDNA 为模板, 用引物 PrimerF 和 PrimerR 进行 PCR 扩增,获得一条约 550 bp 的条带, 与预期大小相似,将目的条带进行凝胶回收,随后 连接至 pMD18-T 载体上,转化大肠杆菌 TOP10.从 转化平板上随机挑取 5 个白斑进行菌液 PCR...
Cross-intron primers were designed according to the nucleotide sequence of the Actin of Phalaenopsis,and Actin homology fragments from Cymbidium sinense and C.goeringii were obtained by reverse transcription polymerase chain reaction(RT-PCR) and PCR using the first chain of cDNA and the genomic DNA...
英文名称:螔-ACTIN upstream and downstream primers (10螠M) (reverse transcription PCR, suitable for human, rat, mouse) 英文同义词: CAS号: 分子式: 分子量:0 EINECS号: Mol文件:Mol File Β-ACTIN上、下游引物(10ΜM)(反转录PCR, 适用于人、大鼠、小鼠) 信息错误报告 ...
Primers of phosphate-starvation-inducible (PSI) genes used for RT-PCR analysis Gene OsPHR1 OsPHR2 OsIPS1 OsIPS2 SQD2 OsPAP10 OsActin Primer sequence (5’-3’) F:CACAAGAAGGGAAAACTACCGATG R:TCAAGATTCATGCACTCTACGACGC F:CGCTTTGTAGATGCTGTCAATC R:AGACCCTCATCACATCCTCATTATC F:AAGGGCAGGG...
对照品不仅简单易用,而且与两步法 RT-PCR 的通用条件完全兼容。只需加入 TaqMan™ 通用 PCR 预混液(含或不含 AmpErase™ UNG)和您的 cDNA 样本,即可在 Applied Biosystems 仪器(包括 Applied Biosystems 7900HT、7300、7500 实时荧光定量 PCR 系统以及 7000 和 7700 序列检测系统)上产生高度灵敏、可重现和...
In this research, through a method of homology cloning, a pair of degenerate primers were designed based on the conserved sequences of Actin genes from other plants submitted to GenBank, and then 4 cDNA fragments from Cinnamomum camphora were obtained using RT-PCR. Molecular biological analysis ...
Interestingly, although quantitative real-time-PCR (Q-RT-PCR) confirmed that single siRNA transfection reduced mRNA for SPIRE1 and SPIRE2, only SPIRE1 knockdown resulted in a significant decrease in the proportion of cells with dispersed melanosomes compared with control (NT) siRNA-transfected cells...