The software AutoPrime automates the task of generating such primers for RT-PCR experiments by combining the information from sequence databases with primer design software. Thus the software is able to cope with the high demand of RT-PCR validation experiments following the recent increase in ...
Design of primers and probes for quantitative real-time PCR methods. Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an a... A Rodríguez,M Rodríguez,JJ Córdoba,... - 《Methods Mol ...
(2003) Genome-scale design of PCR primers and long oligomers for DNA microarrays. Nucleic Acids Res., 31, 5576-5581.Haas, S.A.; Hild, M.; ... SA Haas,H Marc,APH Wright,... - 《Nucleic Acids Research》 被引量: 82发表: 2003年 DNA microarrays with PAMAM dendritic linker systems ...
进入CircInteractome主页(https://circinteractome.nia.nih.gov/),选择Divergent primers search;输入circRNA分子后,点击Divergent primers search,即可获得所查询circRNA分子的junction sequence,下面还附带了2个PCR引物设计工具primer3和NCBI primer design. 实操如下: siRNA干扰序列设计: CircInteractome将会提供10个候选si...
A primer pair for amplifying part of the genome of all potyvirids by RT-PCR RT-PCRPoty-Rymo-Bymo-Ipomo-Macluravirus identificationSequence analysis was used to design a pair of degenerate oligonucleotide primers that amplified a 1.6... GA Mackenzie - 《Journal of Virological Methods》 被引量...
A Unique Primer with an Inosine Chain at the 5'-Terminus Improves the Reliability of SNP Analysis Using the PCR-Amplified Product Length Polymorphism Method. Here, we report allele-specific primers with an inosine chain at the 5'-terminus for PCR-APLP analysis. This unique design improves the...
Real-time PCR Primer And Probe Design Quantitative Reverse Transcription PCR Antibodies Protein Experimental Models Kits Please check back later as we are actively working on gathering the necessary information. Thank you for your understanding and patience. ...
The band of interest is excised, placed into a Gel Nebulizer(TM) inside a Micropure(TM) separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.关键词:...
We offer options for virtually any application and delivery times to keep your research moving. Whether you need oligos for PCR, cloning, hybridization probes, or sequencing sample prep, Invitrogen®, Applied Biosystems®, and TaqMan® custom oligo synthesis services offer the quality and ...
Design a custom 96-well or 384-well PCR plate. Configure New Plate More Assay Search Tools Find a PrimePCR Array Find a predesigned PCR plate for your biological pathway or disease of interest. Customize PCR Primers or Probe Assays