Here we show that the genetic ablation of INO80C activity leads to partial YCS resistance, suggesting that elevated levels of nuclear G-actin may stimulate INO80C to increase DNA polymerase processivity and convert single-strand lesions into double-strand breaks....
In lamellipodia, cortical flow is driven by the combination of Arp2/3-dependent actin assembly at the leading edge and myosin II-based contractility at the bases of lamellae.17 Consistent with this, we found that cortical flow was reduced by blebbistatin, CK666, and jasplakinolide (BCJ) when ...
4.2. Actin Networks and Aggregate Coalescence We previously showed that older mothers form multiple aggregates that fail to coalesce compared to their corresponding young daughters [42]. Furthermore, mature propagating [PSI+] aggregates have been shown to use actin/myosin networks to localize to IPO...
Figure 1.Labeled tropomyosins bind cooperatively to actin filaments. (A) Schematic illustration of Tpms preparation, labeling and functional evaluation. (B) Diagram of Tpm1.1 (αTm) and Tpm1.6 (Tm2) isoforms. (C) Cysteine residues at position 190 on the recombinant tropomyosin dimers were label...
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This is an important functional property because only filamentous actin can transmit tension and effectively activate the myosin Atp ase with the concomitant transformation of chemical energy (ATP hydrolysis) in mechanical work. The in vitro polymerization of muscle actin can be obtained in the ...
Because the phallotoxin derivatives are relatively small, with approximate diameters of 12–15 Å and molecular weights below 2000 daltons, a wide variety of actin-binding proteins—including myosin, tropomyosin, troponin and DNase I—can still bind to actin after tr...
Dock4 is regulated by RhoG and promotes Rac-dependent cell migration. Exp. Cell Res. 312, 4205–4216 (2006). Article CAS PubMed Google Scholar O’Neill, A. K. et al. Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation. J. Cell Biol....
I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem. 246, 4866–4871 (1971). CAS PubMed Google Scholar Bombardier, J. P. et al. Single-molecule visualization of a formin-capping protein ‘decision ...
Actin polymerization locally increases at the furrow cortex during cytokinesis in response to RhoA signaling [13]. Actin dynamics deeper in the cytoplasm are less well investigated, in part because cortical actomyosin is thought to dominate the mechanical behavior of small cells. Keratin and vimentin...