EisakuKatayamaWakabayashi, T, Toyoshima, C, Katayama, E Image analysis of the complex of actin- tropomyosin and myosin subfragment 1. In: Pollack, GH, Sugi, H eds. (1984) Contractile Mechanisms in Muscle. Plenum, New York, pp. 21-26...
of individual functioning molecules, such as myosin crossbridges underin vitromotility assay conditions. With that method, we could actually show that both heads of heavy meromyosin (HMM) crossbridges are mostly straight and bound to actin filaments with about 45 degree tilt-angle under rigor ...
Tilt series are commonly used in electron tomography as a means of collecting three-dimensional information from two-dimensional projections. A common problem encountered is the projection alignment prior to 3D reconstruction. Current alignment technique
We defined a backbone dataset created from imaging a combination of seven different organelle types (Actin, ER, Golgi, Lysosome, Matrix-labelled Mitochondria, Microtubule, and outer membrane-labelled Mitochondria). Three fine-tuning datasets were created from three different samples, acquired from three...
tion, using Jasplakinolide and cytochalasin D to stabilize and desta- bilize the actin cytoskeleton, respectively (Figure 5a–c). We targeted the interaction of actin and myosin using blebbistatin (non-muscle myosin II inhibitor preventing cell contraction allowing blebbing) and ML-7 (a selective ...
Myosins and kinesins are molecular motors that hydrolyse ATP to track along actin filaments and microtubules, respectively. Although the kinesin family inc... AL Wells,AW Lin,L -Q Chen,... - 《Nature》 被引量: 1276发表: 1999年 Surface pore structures of micro- and ultrafiltration membranes...
Panic, R., Ben Messaoud, R., Velut, Y., Hamada, S., Pionneau, C., Duarte, K., Poëa‐Guyon, S., Barnier, J., Nait Oumesmar, B., Bouslama‐Oueghlani, L., 2024.Antagonistic actions of PAK1 and NF2 /Merlin drive myelin membrane expansion in oligodendrocytes. Glia 72...
Structure of the F-actin-tropomyosin complex Filamentous actin (F-actin) is the major protein of muscle thin filaments, and actin microfilaments are the main component of the eukaryotic cytoskeleton. Mutations in different actin isoforms lead to early-onset autosomal dominant non-s... JVD Ecken,...
In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as ai...
7 imaged the subcortical cytoskeleton in living neurons, using the commercially available SiR-actin probe and antibodies carrying the dye STAR580 to label extracellular fragments of neuronal proteins. D’Este et al. demonstrate excellent image quality but report only single frames. Our new approach ...