W. 1989. Analysis of cell division using fluorescently labeled actin and myosin in living PtK2 cells. Cell Motil. Cytoskeleton 14, 201-219.Sanger JM, Mittal B, Dome JS, Sanger JW (1989a) Analysis of cell division using fluorescently labeled actin and myosin in living PtK2 cells. Cell ...
Based on the demonstration that myosin-10 (Myo10) is important for assembly of meiotic spindles, we assessed the role of this unconventional myosin, as well as F-actin, in mitotic spindles. We find that Myo10 localizes to mitotic spindle poles and is essential for proper spindle anchoring, ...
actin and the actin nucleation complex Arp2/3. We found that myosin 1b controls the formation of secretory granules and the associated regulated secretion in both neuroendocrine cells and chromogranin A-expressing COS7 cells used as a simplified model of induced secretion. We show that F-actin ...
Myosin and heavy meromyosin have been spin labeled at either the S 1 or S 2 thiol groups, and their interaction with F-actin has been studied by electron spin resonance, both in the absence of substrate and during the hydrolysis of ATP. ... JC Seidel - 《Archives of Biochemistry & Biop...
The catabolic action of glucocorticoids on the molecular level of the two main muscular proteins, myosin and actin, was found to depend on the type of muscle fibres. The synthesis rate of actin and myosin heavy chain was decreased in all types of muscle fibres, and in myosin light chain onl...
In all doubly labeled isoforms, upon S1 binding, one donor-acceptor "apparent" distance increased slightly by 0.5–2, whereas the other decreased by 6–9. These changes are consistent with a uniform "rolling" motion of Tm over the F-actin surface. The analysis indicates that Tm occupies ...
In cells, actin filaments are organized into cross-linked, branched, and bundled networks. These different architectures appear in structures, such as filopodia, stress fibers, the cell cortex, and contractile actomyosin rings; each has unique physical properties and fulfills different roles in import...
The binding of myosin subfragment-1 (S-1) to F-actin in the absence of nucleotide was examined by the sedimentation method using 1,5-IAEDANS-labeled S-1. W... H Yoshimura,K Mihashi - 《Journal of Biochemistry》 被引量: 0发表: 1982年 Kinetic studies of the cooperative binding of sub...
The acroplaxome is an F-actin/keratin-containing scaffold plate with a marginal ring fastening the caudal recess of the developing acrosome to the nuclear envelope during spermatid nuclear shaping. In contrast to the acroplaxome, fluorescently labeled phalloidin does not produce an obvious F-actin...
Muscle contraction, which is initiated by Ca2+, results in precise sliding of myosin-based thick and actin-based thin filament contractile proteins. The interactions between myosin and actin are finely tuned by three isoforms of myosin binding protein-C