W. (1989) Analysis of cell division using fluorescently labeled actin and myosin in living PtK2 cells. Cell Motil. Cytoskel 14: pp. 201-219Sanger, JM, Mittal, B, Dome, JS, Sanger, JW 1989 Analysis of cell division using fluorescently labelled actin and myosin in living PtK2 ...
ACTIN and myosin have been found in many non-muscle cells. They are presumed to function in the generation of the force required in the various expressions... BURRIDGE, K.,PHILLIPS, J. H. - 《Nature》 被引量: 209发表: 1975年 Fluorescence anisotropy of labeled F-actin: influence of dival...
actin and the actin nucleation complex Arp2/3. We found that myosin 1b controls the formation of secretory granules and the associated regulated secretion in both neuroendocrine cells and chromogranin A-expressing COS7 cells used as a simplified model of induced secretion. We show that F-actin ...
We have visualized contractile proteins, including myosin, filamentous actin (F-actin), alpha-actinin, tropomyosin, and vinculin in human neutrophils during movement by fluorescence microscopy using double immunofluorescence staining procedures of fluorescein isothiocyanate-labeled antibodies, rhodamine-labeled ...
Studies in Drosophila have identified the actin-based molecular motor protein Myosin1D (Myo1D, a.k.a. Myosin31DF) as an essential regulator of LR asymmetry20,21. LR asymmetry in Drosophila governs the dextral rotation of male genitalia, as well as the looping of testis, midgut and hindgut....
Figure 4. Vesicle Exit from the Synapse and Fast Long-Range Motility Are Activity Dependent (A) A cartoon model showing labeled vesicles exiting from the synapse at a constant rate (ζ) that are replaced by un-labeled vesicles at the same rate (χ). Over time, reduction in labeled vesicles...
In all doubly labeled isoforms, upon S1 binding, one donor-acceptor "apparent" distance increased slightly by 0.5–2, whereas the other decreased by 6–9. These changes are consistent with a uniform "rolling" motion of Tm over the F-actin surface. The analysis indicates that Tm occupies ...
(FN3)-like domains and eight immunoglobulin-like domains that can form links between the thick and thin filaments9. Biochemical investigations support a model in which the amino-terminal region can bind to either myosin heads or actin in a phosphorylation-dependent manner10, whereas the C-terminal...
The acroplaxome is an F-actin/keratin-containing scaffold plate with a marginal ring fastening the caudal recess of the developing acrosome to the nuclear envelope during spermatid nuclear shaping. In contrast to the acroplaxome, fluorescently labeled phalloidin does not produce an obvious F-actin...
and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor 488, eBioscience™ (Product # 53-6503-82) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight (Panel a: Green). Nuclei (Panel b: Bl...