本研究作者首先从已确诊的RA患者中提取FLSs,验证NAT10基因及蛋白的表达并检测整体修饰水平。同时,利用acRIP-seq&RNA-seq联合分析鉴定出NAT10的潜在靶点,采用RIP-qPCR实验验证NAT10与靶基因mRNA的相互作用。结果发现RA患者FLSs和滑膜中NAT10和ac4C水平升高,NAT10敲除或特异性抑制剂治疗可减少RA FLSs的迁移和侵袭,且滑...
qRT-PCR和Western blotting实验验证了NAT10敲低的HT-29和LoVo细胞中FSP1表达降低,而在NAT10过表达的SW-480和DLD-1细胞中观察到FSP1表达上调。此外,利用PACES工具预测发现FSP1 mRNA开放阅读框中含有一个高度保守的ac4C乙酰化位点。RIP-qP...
(A)qRT-PCR分析缺血再灌注和心脏模拟手术中NAT10 mRNA水平; (B)WB分析I/R和心脏模拟手术中NAT10蛋白水平; (C)荧光素酶检测HEK293细胞中激活NAT10启动子的p53质粒; (D)p53与NAT10启动子结合的ChIP分析; (E)WB检测H/R感染心肌细胞中NAT10蛋白水平; (F)qRT-PCR分析p53质粒心肌细胞中NAT10 mRNA水平; (G)...
本试剂盒包含了MeRIP流程中的所有核心试剂,如:验证过的ac4C抗体、RNA片段化试剂、蛋白A/G磁珠、IP和洗脱缓冲液等,具有更好的性价比。此外,试剂盒中包含了对照IgG抗体,便于用户进行实验质控,保证了实验的严谨性。优化过的实验流程极其简洁和高效,富集得到的ac4C RNA可以广泛应用于后续的定量RT-PCR、高通量测序以及其...
此外,试剂盒中包含了对照IgG抗体,便于用户进行实验质控,保证了实验的严谨性。优化过的实验流程极其简洁和高效,富集得到的ac4C RNA可以广泛应用于后续的定量RT-PCR、高通量测序以及其它检测分析。 GenSeq® ac4C IP试剂盒 试剂盒别名 ac4C RNA乙酰化免疫沉淀试剂盒...
ac4CRNA修饰相关酶PCR芯片 寻找上游直接调控ac4C RNA乙酰化的甲基转移酶。 04 ac4C RNA修饰靶基因验证 acRIP-qPCR 云序提供acRIP-qPCR服务,可针对mRNA,lncRNA,环状RNA等不同类型的RNA分子进行检测,低通量验证靶基因RNA修饰水平。 05 机制互作研究 RIP-seq/qPCR ...
ac4c RIP试剂盒GenSeq(R) ac4C RIP试剂盒专为RNA ac4C研究而设计,通过MeRIP方法,可以实现对转录组中ac4C修饰区域的特异性富
RIP-qRT-PCR analysis identified an interaction between NAT10 and NR2F1 mRNA in NEP cells and NR2F1 accelerated the nucleus-to-cytoplasm translocation of yes-associated protein 1, which contributed to ectodermal differentiation of hESCs. Collectively, these findings point out the novel regulatory ...
Thus, qRT-PCR was conducted to evaluate the effect of down-regulating the expression of NAT10 on the expression of multiple glycolysis-related genes (Fig. 6B, C). Given that the enhancement of tumor glycolysis can inhibit the activation of various immune cells in the immune microenvironment, ...
NAT10 stimulates the expression of KIF23 through ac4C modification.AThe mRNA level of KIF23 detected by qRT-PCR in 80 CRC tissues and matched adjacent tissues.BCorrelation analysis between the mRNA levels of NAT10 and KIF23.CNAT10 RIP followed by qPCR in SW480 and HT-29 cells.DacRIP follow...