本研究作者首先从已确诊的RA患者中提取FLSs,验证NAT10基因及蛋白的表达并检测整体修饰水平。同时,利用acRIP-seq&RNA-seq联合分析鉴定出NAT10的潜在靶点,采用RIP-qPCR实验验证NAT10与靶基因mRNA的相互作用。结果发现RA患者FLSs和滑膜中NAT10和ac4C水平升高,NAT10敲除或特异性抑制剂治疗可减少RA FLSs的迁移和侵袭,且滑...
(A)qRT-PCR分析缺血再灌注和心脏模拟手术中NAT10 mRNA水平; (B)WB分析I/R和心脏模拟手术中NAT10蛋白水平; (C)荧光素酶检测HEK293细胞中激活NAT10启动子的p53质粒; (D)p53与NAT10启动子结合的ChIP分析; (E)WB检测H/R感染心肌细胞中NAT10蛋白水平; (F)qRT-PCR分析p53质粒心肌细胞中NAT10 mRNA水平; (G)...
GenelilyTMac4C RIP试剂盒专为RNA ac4C研究而设计,通过MeRIP方法,可以实现对转录组中ac4C修饰区域的特异性富集。本试剂盒包含了MeRIP流程中的所有核心试剂,如:验证过的ac4C抗体、RNA片段化试剂、蛋白A/G磁珠、IP和洗脱缓冲液等,具有更好的性价比。此外,试剂盒中包含了对照IgG抗体,便于用户进行实验质控,保证了实验...
qRT-PCR和Western blotting实验验证了NAT10敲低的HT-29和LoVo细胞中FSP1表达降低,而在NAT10过表达的SW-480和DLD-1细胞中观察到FSP1表达上调。此外,利用PACES工具预测发现FSP1 mRNA开放阅读框中含有一个高度保守的ac4C乙酰化位点。RIP-qP...
ac4c RIP试剂盒GenSeq(R) ac4C RIP试剂盒专为RNA ac4C研究而设计,通过MeRIP方法,可以实现对转录组中ac4C修饰区域的特异性富
ac4C RIP Kit ac4C RNA Enrichement Kit ac4C Immunoprecipitation Kit ac4C IP Kit 产品优势 信噪比更优:优化过的实验体系,验证过的抗体,信噪比优 流程更严谨:包含对照IgG抗体,方便实验质控 性价比更高:包含ac4C MeRIP过程中的所有**试剂,性价比高 实验流程 ...
ac4CRNA修饰相关酶PCR芯片 寻找上游直接调控ac4C RNA乙酰化的甲基转移酶。 04 ac4C RNA修饰靶基因验证 acRIP-qPCR 云序提供acRIP-qPCR服务,可针对mRNA,lncRNA,环状RNA等不同类型的RNA分子进行检测,低通量验证靶基因RNA修饰水平。 05 机制互作研究 RIP-seq/qPCR ...
RIP-qRT-PCR analysis identified an interaction between NAT10 and NR2F1 mRNA in NEP cells and NR2F1 accelerated the nucleus-to-cytoplasm translocation of yes-associated protein 1, which contributed to ectodermal differentiation of hESCs. Collectively, these findings point out the novel regulatory ...
D, E The interaction between NAT10 and JunB mRNA was analyzed by RIP-qPCR assay in TNBC cells with NAT10 knockdown or overexpression. F, G mRNA and protein level of JunB upon NAT10 knockdown or overexpression. H, I RNA decay experiment validated JunB mRNA stability upon NAT10 knockdown or...
(Fig.3B). Moreover, PCR showed that LINC00623 could be detected in the RNA immunoprecipitation (RIP) products using an antibody against NAT10 (Fig.3C). In addition, the results of the immunofluorescence (IF) assays confirmed the colocalization of LINC00623 and NAT10 (Fig.3D). Next, a...