图二显示了不同AAV血清型的中和抗体(Neutralizing Antibody, NAb)和抗药抗体(Anti-Drug Antibody,ADA)的流行率[10]。中和抗体属于抗药抗体,中和抗体一方面会阻碍AAV载体进入细胞发挥药效,另一方面会引起严重的免疫毒性。中和抗体的分析根据药物作用机制和靶点选择合适的检测方法。根据是否使用细胞分为基于细胞试验和基于...
8.Guo, Ping, et al. "Rapid AAV-neutralizing antibody determination with a cell-binding assay." Molecular Therapy-Methods & Clinical Development 13 (2019): 40-46.9. Shao, Wenwei, et al. "Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduc...
最常使用的AAV2和AAV9的血清阳性率分别为约59.0%和33.5%。图二显示了不同AAV血清型的中和抗体(Neutralizing Antibody, NAb)和抗药抗体(Anti-Drug Antibody,ADA)的流行率[10]。中和抗体属于抗药抗体,中和抗体一方面会阻碍AAV载体进入细胞发挥药效,另一方面会引起严重的免疫毒性。...
Baatartsogt, N. et al. A sensitive and reproducible cell-based assay via secNanoLuc to detect neutralizing antibody against adeno-associated virus vector capsid. Mol. Ther. - Methods Clin. Dev. 22, 162–171 (2021).
Baatartsogt, N. et al. A sensitive and reproducible cell-based assay via secNanoLuc to detect neutralizing antibody against adeno-associated virus vector capsid. Mol. Ther. - Methods Clin. Dev. 22, 162–171 (2021).
AAV VECTOR, AND ASSAY FOR ANTI-AAV (ADENO-ASSOCIATED VIRUS) NEUTRALIZING ANTIBODIESTo provide virus vectors, virus particles, and methods and uses of screening, detecting, analyzing and determining amounts of virus antibody, or neutralizing antibody activity of samples.SOLUTION: The virus vectors, ...
Baatartsogt, N. et al. A sensitive and reproducible cell-based assay via secNanoLuc to detect neutralizing antibody against adeno-associated virus vector capsid. Mol. Ther. - Methods Clin. Dev. 22, 162–171 (2021).
Complement activation by adeno-associated virus-neutralizing antibody complexes. Hum Gene Ther. 2023;34:554–66. Article CAS PubMed PubMed Central Google Scholar Elmore ZC, Oh DK, Simon KE, Fanous MM, Asokan A. Rescuing AAV gene transfer from neutralizing antibodies with an IgG-degrading ...
AAV neutralizing antibody assay To determine if intravitreal injection of AAV vectors results in an antibody response to the injected capsid, neutralizing antibody assays were performed on HEK 293 cells using a previously reported method14. In short, cells were seeded in a 48-well plate at 25,000...
(e.g., in mice), and total antiviral capsid antibody (TAb) tests based on enzyme-linked immunosorbent assays (ELISA). TAb methods can detect inefficient NAb below the threshold for TI detection, but may not detect non-antibody neutralizing factors. In vivo and in vitro ...