aIsotypes and neutralization of anti-CVA16 MAbs (8C4, 9B5, and 9G1). Neutralization concentration was defined as the lowest antibody concentration that fully prevented cytopathic effect. Neutralization IC50 of each MAb against CVA16 was determined by the cell viability assay.bNeutralization of the...
Monoclonal antibody biotherapeutics usually have long half-lives and for high-dose indications such as oncology, high level of drug will be present in the testing samples and interfere with ADA and/or neutralization antibody (NAb) measurement. To overcome this drug interference, acid-dissociation-...
We further describe how to use the collected plasma to measure the quantity and quality of B2Δ-specific antibodies by ELISA or toxin neutralization assay. This protocol also describes a method to measure B2Δ-specific plasma cells by ELISPOT assay. Our overall strategy can be adapted to other ...
Architecture and antigenicity of the Nipah virus attachment glycoprotein. PMID: 35239409 Potent monoclonal antibody-mediated neutralization of a divergent Hendra virus variant. PMID: 35617431 A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses. PMID: 3...
SN/T 1181.2-2003 Detection antibodies against foot and mouth disease virus-Protocol of micro-serum neutralization testOthers: neutralizing antibody, Neutralizing antibody detection, Neutralizing antibody detection, neutralizing antibody cells, neutralizing antibody, neutralizing antibody antibody, trace ...
Neutralization assay of recombinant human IL-19 stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 Rß in a dose-dependent manner (orange line). Proliferation elicited by recombinant human IL-19 (3 ng/mL) is neutralized (green line) ...
(T332N)9was used to measure autologous neutralization for the majority of experiments. The SHIV.BG505.332N.375Y virus stock, prepared by transfection into HEK293T cells, provided by G. Shaw was used directly to assess autologous neutralization. The TZM-bl neutralization assay used has been ...
protocol.6The assay targets multiple variable gene segments in rearranged immunoglobulin andTCR genes. The primers are designed to probe three VH-JH regions, two DH-JH regions, two IGK regions, one IGL region, three TCRβ regions, two TCRγ regions, and one TCRδ region. This protocol ...
To measure bioactivity, interferon was titrated with the use of a cytopathic effect inhibition assay, where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha to a 50% endpoint. In this antiviral assay, approximately one unit/mL of ...
Therefore, an integrated analysis of pharmacokinetic, pharmacodynamic, and immunogenicity data to interpret neutralizing activity of low risk biotherapeutics has been proposed by the industry as an alternative approach in understanding any neutralization of therapeutic activity (14, 15). The decision to ...