[9] Zen Z, Espinoza Y, Bleu T, Sommer JM, Wright JF. Infectious titer assay for adeno-associated virus vectors with sensitivity sufficient to detect single infectious events. Hum. Gene Ther. 2004. 15(7), 709–715. [10] BLA Clinical Review Memorandum (Luxturna). FDA. 2017. ...
[8] US FDA, Center for biologics evaluation and research. Guidance for industry: Design and Analysis of Shedding Studies for Virus or Bacteria-Based Gene Therapy and Oncolytic Products. 2015 [9] Zen Z, Espinoza Y, Bleu T, Sommer JM,...
[9] Zen Z, Espinoza Y, Bleu T, Sommer JM, Wright JF. Infectious titer assay for adeno-associated virus vectors with sensitivity sufficient to detect single infectious events. Hum. Gene Ther. 2004. 15(7), 709–715. [10] BLA Clinical Review Memorandum (Luxturna). FDA. 2017. [11] Phar...
AAV Infectious Titer Assay Endotoxin Assay Services Replication-Competent AAV Testing Residual Host-cell DNA Testing AAV Transduction Efficiency Assay AAV Viral Protein Purity Testing AAV Vector Toxicity and Safety Assay AAV Aggregation Assay Recombinant adeno-associated viral (rAAV) vectors have been used...
S. Zolotukhin Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield Gene Ther., 6 (1999), pp. 973-985 CrossrefView in ScopusGoogle Scholar Cited by (186) Combinatorial peptide libraries: Mining for cell-binding peptides 2014, Chemical Reviews In ...
The physical titer is the concentration of viral particles containing viral genomes but this may differ to the infectious titer which is the concentration of viral particles that can transduce cells. So the ratio of physical to infectious viral particles indicates the specific infectivity of AAV ...
Below a gc-titer of 1 × 109 the expression of GFP was between 0.35% and 0.01%. Cells in neither of the control conditions did express GFP (data not shown). Figure 3 In vitro infectivity assay. Results from the in vitro transduction of HeLa cells for decreasing gc AAV-GFP vector ...
assay kit according to the manufacturer’s instructions. Concentration of intact particles was calculated based upon 1 μg is equivalent to 2.7 × 1010genome intact particles36. The ratio of intact adenoviral particles, determined by the PicoGreen assay, and the Tissue Culture Infectious Dose ...
the cells were incubated at 37° C. with 5% CO2for 5 days and another layer of plaque assay solution was overlaid on the dish to feed the cells. The plaques started to appear around one week after the initial infection and reached a plateau at two weeks. The final titer was determined...
While AAV is a powerful vehicle for gene delivery, unstable ITRs threaten the fidelity of rAAV genomes. Loss of ITR integrity diminishes the packaging efficiency of rAAV particles and thus viral titer. In this tech note, discover the effect of partial and full ITR deletions on AAV packaging ...