This assay was conducted with the strains disaplying the highest and the lowest susceptibility to endolysin. All the strains tested were susceptible to colistin (TableS1), except three strains, which were colistin resistant:A. baumanniiSOF004b,P. aeruginosaAUS034 andK. pneumoniaeKP2. The effect ...
The shortcomings of this so-called in vitro testing are obvious: substances which lack an effect on the viral replication cycle but, e.g., enhance antibody formation, improve nonspecific resistance, strengthen the interferon systems will not be detected by this method. Likewise, although so far ...
While functional effects such as the ion-channel assays are important, adverse structural effects can also be critically detrimental. A recent in vitro assay using cardiomyocytes derived from stem cells is claimed to be capable of assessing both structural and functional damage in the same assay, pr...
Despite testing a limited number of compounds in vitro (n = 10), we were able to identify four compounds with previously unknown activity against SARS-CoV-2. These active compounds showed 18–40% viral inhibition using an established live virus infection assay. While their activity is low when...
In vitroandin vivoefficiency of our method of transfection was assessed by transfection of EL4-IL-2 thyoma cells and mice with p21-GFP construct, respectively. The authenticity of p21-GFP construct is shown in Figure2A. For in vitroassay, cells were transfected with p21-GFP construct and cell...
the flow-through and wash fractions were determined by Gag-p24 antigen ELISA and by titration of 20-2.5 μl in a 2-fold serial dilution in a standard infection assay with LC5-RIC cells. The virus capture assays based on binding of viral gp120 envelope protein to heparin-beads were ...
Poster : Enhancing AAV productivity through Helper plasmid design and plasmid ratio optimization Infographic : Polyplus solutions for viral vector production Infographic : GMP AAV manufacturing process using transient transfection in a 2 000 L bioreactor (Theoretical approach) View All Resources...
20892AbstractViralvaccinesandthecellsubstratesusedtomanufacturethemaresubjectedtotestsforadventitiousagents,includingviruses,whichmightcontaminantthem.Someofthecompendialmethods(invivoandinvitroincellculture)wereestablishedinthemid-20thcentury.Thesemethodshavenotbeensubjectedtocurrentassayvalidation,asnewmethodswouldneedtobe...
Fig. 1: Construction, map and confirmation of the CRISPR-Cas9 construct targeting SIV proviral DNA in vitro. aSchematic presentation of the full-length SIVmac239 genome (GenBank: M33262.1) with positions of targeted sites, predicted cleavage events and gRNAs sequences.bMap of AAV pX601-SaCas9...
A quantitative RT-PCR assay was performed to assess TNF-α siRNA levels in sEVs derived from the culture medium of primary hepatocytes (n = 4 in each group). Values are presented as the mean ± SEM. Significance was determined using one-way ANOVA followed by Dunnett’s multiple ...