VRC) 的John R. Mascola实验室和Peter D. Kwong实验室从一名HIV感染者体内首次分离出一种主要结合HIV Env上保守的融合肽 (Fusion peptide, FP) 区域的广谱中和抗体 (Broadly neutralizing antibody, bNAb) , VRC34.01抗体【4】。接
VRC)的John R. Mascola实验室和Peter D. Kwong实验室从一名HIV感染者体内首次分离出一种主要结合HIV Env上保守的融合肽(Fusion peptide, FP)区域的广谱中和抗体(Broadly neutralizing antibody, bNAb), VRC34.01抗体【4】。接
cell-based NAb assaydrug tolerancematrix interferencemodality containing endogenous homologysample pretreatmentTherapeutic protein drugs can potentially induce immune responses in patients and result in the production of anti-drug antibodies (ADAs), including a subset of ADAs called neutralizing antibodies (...
Cell SnapShot重磅推出广谱中和抗体(broadly neutralizing antibody, bnAb)。详细解读了bnAb的定义以及各种病毒存在的诱导中和抗体的表位。 理想的疫苗应诱导出能够中和绝大多数甚至所有正在传播的此类病毒家族的抗体,这样的抗体被称为广谱或广泛中和抗体(broadly neutralizing antibody,bnAb)。尽管它们的特征和 功能 有所...
近日,杜克大学医学院等机构的科学家们在Cell上发表题为“Vaccine induction of CD4-mimicking HIV-1 broadly neutralizing antibody precursors in macaques”的研究论文,成功研发HIV疫苗的途径依赖于关键的第一步,即激活特定免疫细胞来诱导广谱中和性抗体的产生。
The results demonstrate feasibility of the strategy using the integrated vector system that allows use of scFv-Fc as a reliable format for rapid cell-based antibody screening and validation. Results Design features of the integrated vector system Here we used two vectors, pDR-D1 (Figure 1A) for...
已经有非常多的研究揭示了SARS-CoV-2感染的致病机理和免疫逃逸的机制,其中最为大家熟知的就是spike蛋白的突变导致各种病毒变种的出现,spike蛋白是病毒与人体细胞受体结合的关键部分,其突变通常会影响病毒的传染性、致病性以及疫苗的有效性【2-4】。同时,人体免...
The current SARS-CoV-2 variants strikingly evade all authorized monoclonal antibodies and threaten the efficacy of serum-neutralizing activity elicited by vaccination or prior infection, urging the need to develop antivirals against SARS-CoV-2 and relate
Neutralizing antibody assay by the Emory Vaccine Center The HIV-1 Env clone BG505.W6M.Env.C2 (GenBank accession codeDQ208458) that had been modified to contain the N332 glycan (T332N)9was used to measure autologous neutralization for the majority of experiments. The SHIV.BG505.332N.375Y vir...
Here we describe generation of stable, fluorescent protein-barcoded reporter cell lines suitable for multiplex screening of antibody to membrane proteins. The utility of this cell-based system, with the potential of a 256-plex cell panel, is demonstrated by flow cytometry deconvolution of barcoded ...