Cell Type细胞类型:表皮 Morphology形态: Culture Properties培养特性: Disease疾病:肺癌 Biosafety Level生物安全级别: Age年龄:58 Gender性别:男 Applications应用: Images图片: Complete Growth Medium完全培养基: Subculturing传代方法: Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5%...
(DepartmentofRespiratoryDiseases,SouthwestHospital,ThirdMilitaryMedicalUniversity,Chongqing,400038,China)【Abstract】ObjectiveToisolateandidentifythelungcancerstemcellsfromtheestablishedhumanlungadenocarcinomacelllineA549.MethodsTheA549cellswereculturedinserum-freeconditionmediumtoformtumorspheres.ColonyformationassayandMTT...
Cell Type细胞类型:Morphology形态:Culture Properties培养特性:Disease疾病:Biosafety Level生物安全级别:Age年龄:Gender性别:Applications应用:Images图片:Complete Growth Medium完全培养基:Subculturing传代方法:Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage ...
Long-termculture of the human lung adenocarcinoma cell line A549 promotes thedifferentiation of these cells toward an alveolar type II cell phenotype. Here,we evaluated the susceptibility of long-term cultured A549 cells to humaninfluenza viruses. A549 cells were cultured continuously for 25days(D25-...
组织来源Tissue and Cell Type 肺部 形态Morphology 上皮样,贴壁生长 培养基和添加剂Complete Growth Medium and Culture Conditions A549细胞完全培养液配方:F-12K(Invitrogen,21127-022) 89 mlFBS(Gibco) 10 mlGlutamax (Invitrogen, 35050061) 1 ml 参考传代周期:每周2-3次参考传代比例:1 : 3备注:培养时保持细...
近日,东京大学医学研究所Yoshihiro Kawaoka等人评估了长期培养的A549细胞对人类流感病毒的易感性。相关研究以Long-term culture ofhuman lung adenocarcinoma A549 cells enhances the replication of humaninfluenza A viruses发表在Journal of GeneralVirology杂志上。
OrganismTissueMorphologyCulture Properties Homo sapiens (Human)LungEpithelialAdherent 本细胞株详细信息如下: General Information Cell Line NameA549 (Human Non-Small Cell Lung Cancer Cells) SynonymsA 549; A-549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 ...
Tissue and Cell Type肺癌 形态 Morphology上皮细胞 培养基和添加剂 Complete Growth Medium and Culture ConditionsF-12K Nutrient Mixture (gibco by life technologies 21127-022),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
2.1Cell culture The human alveolar type II epithelial cell line A549 was purchased from American Type Culture Collection (Manassas, VA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10%fetal calf serum(FCS) and 1% penicillin/streptomycin.A549 cellswere cultured in...
Tissue and Cell Type肺部 形态 Morphology上皮样,贴壁生长 培养基和添加剂 Complete Growth Medium and Culture ConditionsA549细胞完全培养液配方: F-12K(Invitrogen,21127-022) 89 ml FBS(Gibco) 10 ml Glutamax (Invitrogen, 35050061) 1 ml 参考传代周期:每周2-3次 ...