A549 cells are epithelial cells, meaning they are cells that line the surfaces of organs and tissues. A549 adenocarcinoma cells are grown in a laboratory in a nutrient-rich DMEM culture medium. When they are grown in this way, they form a monolayer (single layer of cells). A549 cells becam...
Cell Type细胞类型:表皮Morphology形态:Culture Properties培养特性:Disease疾病:肺癌Biosafety Level生物安全级别:Age年龄:58Gender性别:男Applications应用:Images图片:Complete Growth Medium完全培养基:Subculturing传代方法:Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO ...
Quality Control This cryovial contains at least 1.0 × 106 A549/GFP cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The A549/GFP cells are tested free of microbial contamination. Medium 1. Culture Medium: D-MEM (high glucose), 10% fetal bovine serum (...
The present invention provides a method for preparing (eg, adenovirus) viruses are the A549 cells derived from the adapted for growth in non-animal material-free medium suspension culture and serum-free. The adapted A549 cell line, has the characteristics of cell lines that are identified as (...
Culture Method Doubling Time ~22 hrs Methods for Passages Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) Medium DMEM (high glucose)+...
近日,东京大学医学研究所Yoshihiro Kawaoka等人评估了长期培养的A549细胞对人类流感病毒的易感性。相关研究以Long-term culture ofhuman lung adenocarcinoma A549 cells enhances the replication of humaninfluenza A viruses发表在Journal of GeneralVi...
;生长特性:贴壁生长;形态特性:上皮样;多角形;相关产品有:HIEC-6细胞、HIT T15细胞、Leghorn Male Hepatoma cell line细胞 SBB-W1 Cells(提供STR鉴定图谱) Hs-578Bst Cells;背景说明:乳腺 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MN 60细胞、P3-Ji...
The stable isotope labeling was performed as described previously13. In brief, A549 cells were maintained in SILAC Protein Quantitation Kit (Invitrogen, Carlsbad, CA) according to manufacturer's instructions. Briefly, cells were maintained in DMEM culture medium supplemented with 10% fetal bovine serum...
Required Cell Culture Medium • Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% heat-inactivated fetal bovine serum (FBS; 30 min at 56 °C), 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 µg/ml) • Freezing Medium: DMEM, 4.5 g/l glucose, 10% FBS, 10%...
2.1Cell culture The human alveolar type II epithelial cell line A549 was purchased from American Type Culture Collection (Manassas, VA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10%fetal calf serum(FCS) and 1% penicillin/streptomycin.A549 cellswere cultured in...