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Cell Culture A549 cellswere cultured in 25 mL DMEM supplemented with either 10%fetal bovine serumand 2 mM L-glutamine for complete media, or with no serum for serum-free treatments. Cells were grown in 5% CO2in air-humidified incubator at 37°C. The media was replaced every 2 days, and...
ABSTRACT Long-termculture of the human lung adenocarcinoma cell line A549 promotes thedifferentiation of these cells toward an alveolar type II cell phenotype. Here,we evaluated the susceptibility of long-term cultured A549 cells to humaninfluenza viruses. A549 cells were cultured continuously for 25da...
人肺癌 A549 细胞直接暴露可吸入物质体外试验方法的建立及应用 significant reduction of cell viability at different concentrations of NO2 ( MTT estimated curve: yMTT = 0. 989 - 0. 021xNO2 R2 = 0. 84,P < 0. 01; NUR estimated curve: yNUR = 1. 032 - 0. 026xNO2 R2 = 0. 88,P <...
The inhibitory effect on cell proliferation was determined using the WST-8 colorimetric assay and calculated by comparing the absorption of the culture medium of PM10-exposed cells with that of non-PM10-exposed cells at the indicated time points. *P< 0.05 compared with the control. ...
结论:A549细胞表达的CXCL-8能激活Akt通路,促进hBMSCs增殖和运动。关键词趋化因子-8;人骨髓间充质干细胞;Akt信号通路;细胞迁移 Conditioned media prepared from A549 lung carcinoma cells promotes the proliferation and migration of human bone marrow mesenchymal stem cells* Jiang Yang1, Wang Lulu1, Jin ...
Among them, the aptamer Cell APT-2 and Cell APT-4 showed high affinity and strong selectivity to A549 cell culture media with an equilibrium dissociation constant (Kd) of 8.723卤2.180nM and 7.079卤1.336nM respectively. Moreover, the aptamer Cell APT-2 exhibits strong selectivity to lung cancer...
In the replicative analysis at different growth phases of the isolates in cell culture media, growth capacity at 3 h post-incubation was frequently twice as that at 1 h, and that at early-log phase was frequently higher than that at mid-log phase at both post-incubation times. Adherence ...
Cell culture and transfection A549 cells were cultured in the environment at 37 °C with 5% CO2. And nutrient media components were Dulbecco modified Eagle medium (DMEM), 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS). ...