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Cell Culture A549 cellswere cultured in 25 mL DMEM supplemented with either 10%fetal bovine serumand 2 mM L-glutamine for complete media, or with no serum for serum-free treatments. Cells were grown in 5% CO2in air-humidified incubator at 37°C. The media was replaced every 2 days, and...
Among them, the aptamer Cell APT-2 and Cell APT-4 showed high affinity and strong selectivity to A549 cell culture media with an equilibrium dissociation constant (Kd) of 8.723卤2.180nM and 7.079卤1.336nM respectively. Moreover, the aptamer Cell APT-2 exhibits strong selectivity to lung cancer...
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In the replicative analysis at different growth phases of the isolates in cell culture media, growth capacity at 3 h post-incubation was frequently twice as that at 1 h, and that at early-log phase was frequently higher than that at mid-log phase at both post-incubation times. Adherence ...
近日,东京大学医学研究所Yoshihiro Kawaoka等人评估了长期培养的A549细胞对人类流感病毒的易感性。相关研究以Long-term culture ofhuman lung adenocarcinoma A549 cells enhances the replication of humaninfluenza A viruses发表在Journal of GeneralVirology杂志上。
In reality, the capability of cell killing by plasma was even found to be sensitive to the concentration of fetal bovine serum in the media as well as the storage temperature for the media [16]. However, the detailed mechanisms underlying these effects have not yet been fully identified. An...
Please view the article in the link below for more information regarding the phenotype of this cell line:https://www.culturecollections.org.uk/media/126668/jim-plos-one-article-a549.pdf Please visit the ECACC website for additional documentation and information regarding this cell line:https://ww...
篇Cell media 细胞培养基 1001 Endothelial Cell Medium,500 ml 内皮细胞培养基1101 Smooth Muscle Cell Medium, 500 ml 平滑肌细胞培养基1201 Pericyte Medium, 500 ml 周边细胞培养基1521 Neuronal Medium, 500 ml 神经元细胞培养基1601 Oligodendrocyte Precursor Cell Medium, 500 ml 少突先驱胶质细胞培养...
[U−12C6]-L-lysine for over six generations. The heavy labeling efficiency was evaluated by mass spectrometer analysis to a confirmed >97% labeling efficiency. After that, the cells were further expanded in SILAC media to desired cell number (~5 × 108) in twenty 150 cm2flasks. The ...