A549 is a lung cancer cell line with COSMIC ID: 905949 and Sanger ID: 369, and Cell Line Passport number SIDM00903 (Non-Small Cell Lung Cancer (NSCLC), Adenocarcinoma). The A549 cell line was established in 1972 by D.J. Giard et al, through an explant culture of adenocarcinomic lung ...
Cell Culture A549 cellswere cultured in 25 mL DMEM supplemented with either 10%fetal bovine serumand 2 mM L-glutamine for complete media, or with no serum for serum-free treatments. Cells were grown in 5% CO2in air-humidified incubator at 37°C. The media was replaced every 2 days, and...
5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. lncubate cultures at 37℃.操作流程1.吸走多余培养基,加入3-5mL PBS后轻轻晃动培养瓶润洗细胞; 2.吸干净PBS后加入1mL 0.25%胰酶-0.53mM EDTA,轻轻摇晃培养皿使胰酶浸没细胞表面,培养瓶放37度培养箱消化; (细胞对于消化比较敏...
Preparation of protein extracts The A549 cells were subjected to various treatments and then washed with PBS before being dislodged with a cell scraper. Nuclear protein extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extrac- tion Reagent, according to the manufacturer's protocol (...
Cell culture and CNPs treatment Human lung epithelial A549 cells were routinely maintained in RPMI 1640 medium at pH 7.2, supplemented with 10% inactivated foetal bovine serum and 1% penicillin/streptomycin and grown in humidified atmosphere supplied with 5% CO2in air at 37 °C. A549 cells were...
ltx试剂将质粒 dna转染到 a549细胞中 ‹查看所有转染方案 快速链接 介绍 实验材料和订购信息 a549细胞转染 扩大或缩小转染 介绍 介绍 lipofectamine® ltx试剂采用专有的无动物源性配方,用于将 dna转染到真核细胞中,且具有 低细胞毒性 .本参考文件提供了使用 lipofectamine® ltx试...
产品名称 A549 Whole Cell Lysate 规格或纯度 Carrier Free, Azide Free 产品介绍 The A549 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. A549 cells are used as an in vitro model for a type II pulmonary epitheli...
Then the cells were washed carefully with PBS and trypsinized to produce single-cell suspension. 300 cells from each treatment group were plated into 6-cm cell culture dish respectively and incubated for 10 days. After washed with PBS, a mixture of 6% glutaraldehyde and 0.5% crystal violet ...
We examined the effects of HBOT on A549 cells compared with an immortalized human bronchial epithelial cell line, Beas-2B, followed by the cell-based HBOT protocol (Fig. 5A). HBOT suppressed the growth of A549 cells, not Beas-2B cells, in a time-dependent manner as measured in Fig. 5B....
The cells were treated with AMEAE at different concentrations prior to staining the cells with cell permeability and mitochondrial membrane potential (MMP) dyes. Then, cells were fixed and blocked with 1X blocking buffer according to the manufacture’s protocol. Next, primary cytochrome c antibody ...