The exceptions were a) a variant with a clustering of alanine and methionine residues at one end of the three-α-helix bundle, suggesting a gate structure that can admit ligand molecules; and b) a variant with a single Leu35Ala substitution, suggesting that at select positions, the size of...
The fluorescence quenching experiments and the direct photoaffinity labeling studies provide complementary information and reveal that the anesthetic indeed binds in the designed cavity in the hydrophobic core of the four-α-helix bundle. In addition, the structural and dynamic properties of the four-α...
Restored heptad pattern continuity does not alter the folding of a four-α-helix bundle Restored heptad pattern continuity does not alter the folding of a four-alpha-helix bundle. Nature Struct. Biol. 1:706-716, 1994.Restored heptad pattern... M Vlassi,C Steif,P Weber,... - 《Nature St...
Rationally redesigned variants of the 4-helix-bundle protein Rop are described. The novel proteins have simplified, repacked, hydrophobic cores and yet reproduce the structure and native-like physical properties of the wild-type protein. The repacked proteins have been characterized thermodynamically and ...
1994 . Restored heptad pattern continuity does not alter the folding of a four-α-helix bundle . Nature Struct Biol 1 : 706 – 716 ... GJ Davies - 《Nature Structural Biology》 被引量: 82发表: 1994年 Coiled Coil in the Stalk Region of ncd Motor Protein Is Nonlocally Sustained form a...
Of these, the majority are multispanning helix-bundle proteins that partake in important cellular functions such as photosynthesis and respiration, active and passive transport of ions, metabolites, and macromolecules, signal transduction, and a multitude of enzymatic reactions. In mammalian cells, ...
Self-assembly of a four-helix bundle on a DNA quadruplex. Rosenzweig B A,Hamilton A D. Angewandte Chemie International Edition . 2009Rosenzweig B A,Hamilton A D.Self-assembly of a four-helix bundle on a DNA quadruplex.Angewandte Chemie International Edition. 2009...
Csp1 is a tetramer of four-helix bundles with each monomer binding up to 13 Cu(I) ions in a previously unseen manner via mainly Cys residues that point into the core of the bundle. Csp1 is the first example of a protein that stores a metal within an established protein-folding motif....
Engineering a two-helix bundle protein for folding studies Protein Eng. Des. Sel., 23 (2010), pp. 357-364 CrossrefView in ScopusGoogle Scholar [9] L. Aravind, E.V. Koonin SAP – a putative DNA-binding motif involved in chromosomal organization Trends Biochem. Sci., 25 (2000), pp. ...
Amphipathic alpha-helix bundle organization of lipid-free chicken apolipoprotein A-I. Apolipoprotein A-I (apoA-I), the major protein component of plasma high-density lipoprotein (HDL), exists in alternate lipid-free and lipid-bound states. A... Robert S. Kiss,Cyril M. Kay,Robert O. Ryan ...