1997. A designed four helix bundle protein with native-like structure. Nat. Struct. Biol. 4: 1039-1046.Schafmeister CE , LaPorte SL , Miercke LJW , Stroud RM . 1997 . A designed four helix bundle protein with native-like structure . Nature Struct Biol 4 : 1039 – 1046 ....
We are pursuing a combinatorial approach to understand better the sequence structure-relationship: sorting large libraries of protein variants for structured proteins. We have developed a high-throughput cell-based screen for activity of the well-studied four-helix bundle protein Rop. To collect ...
Monte Carlo studies on equilibrium globu- lar protein folding. II -barrel globular protein models. Bio- polymers 1989; 28: 1059-95.Sikorski A, Skolnick J: Monte Carlo Studies on Equilibrium Globular Protein Folding. III. The Four Helix Bundle. Biopo/y- mers 1989, 28:1097-1113....
Further studies are needed to elucidate how multiple interactions occur in the four-helix proteins and how the regular order of the multiple interactions proceeds. This study revealed for the first-time the 3D structure of a membrane protein to form a continuous strand that could be common in ...
Kwan Jamie,Donaldson Logan.The NMR structure of the murine DLC2 SAM domain reveals a variant fold that is similar to a four-helix bundle.BMC Structural Biology. 2007Kwan, J. J., and Donaldson, L. W. (2007) BMC Struct. Biol. 7 34....
Herein, we discuss how our work relates to the evolutionary selective-advantages realized when symmetrical homo-oligomers evolve into monomers. Moreover, our thermally stable single-chain four-helix bundle protein may provide a robust scaffold for development of new biomaterials. 展开 ...
This hypothesis proposes that signals are transmittednthrough the four-helix bundle via changes in helix helix packing and that the helix packing changes in thenadaptation and protein interaction regions are tightly and antisymmetrically coupled. Specifically, strongnhelix packing in the adaptation region...
Protein ScienceBellesia, G, Jewett, AI, Shea, JE (2011) Relative stability of de novo four-helix bundle proteins: insights from coarse grained molecular simulations. Protein Sci 20: pp. 818-826Bellesia G, Jewett AI, Shea JE (2011) Relative stability of de novo four-helix bundle proteins:...
We have determined the crystal structure of FAT and show that it forms a four-helix bundle that resembles those found in two other proteins involved in cell adhesion, alpha-catenin and vinculin. The binding of FAT to the focal adhesion protein, paxillin, requires the integrity of the helical ...
The stability of proteins is an important factor for industrial and medical applications. Improving protein stability is one of the main subjects in protein engineering. In a previous study, we improved the stability of a four-helix bundle dimeric de nov