Recently, we described the design and structure determination of α 3D, a three-helix bundle protein with a well-packed hydrophobic core. Here, we test the malleability and adaptability of this protein's structure by mutating a small, Ala residue (A60) in its core to larger, hydrophobic side...
Here we investigate the unfolding and folding of the three-helix bundle protein KIX, for which ECD data indicated substantial preservation of the native solution structure in the (M + 7H)7+ions, on a timescale of at least 4 s after transfer into the gas phase, even after vibrational ion ...
The preparation of ±3D, a single polypeptide that folds into a native-like three-helix bundle structure, has significantly expanded available de novo designed scaffolds. Devoid of a metal-binding site (MBS), we incorporated a 3Cys and 3His motif in ±3D to construct a heavy metal and a ...
F. From coiled coils to small globular proteins: design of a native-like three-helix bundle. Protein Sci. 7, 1404–1414 (1998). Article CAS PubMed PubMed Central Google Scholar Tripet, B., Wagschal, K., Lavigne, P., Mant, C. T. & Hodges, R. S. Effects of side-chain ...
Design of a three-helix bundle capable of binding heavy metals in a triscysteine environment. An important objective of de novo protein design is the preparation of metalloproteins, as many natural systems contain metals that play crucial roles for ... S Chakraborty,JY Kravitz,P Thulstrup,......
1) (19). Intriguingly, it was also shown that a lysine-rich unstructured tail preceding the terminal three-helix bundle, although previously shown to be necessary for high affinity recognition (15), does not contact the G-quadruplex in the final complex (see Fig. 1, inset). Download: ...
Dynamics of a De Novo Designed Three-Helix Bundle Protein Studied by [sup 15]N, [sup 13]C, and...Investigates the backbone and side-chain dynamics of de novo designed three-helix bundle protein. Similarity between the alpha[sub 3]D and natural proteins; Observation of the C methyl data ...
α3D-CH3 is three helix bundle peptide that was designed to contain a copper ET site that is found in the β-barrel fold of native cupredoxins. The ET activity of Cuα3D–CH3 was determined using five different photosensitizers. By exhibiting a complete depletion of the photo-oxidant and ...
The solution structure of the amino-terminal HHCC domain of HIV-2 integrase: A three-helix bundle stabilized by zinc Curr. Biol., 7 (1997), pp. 739-746 View PDFView articleView in ScopusGoogle Scholar 21 A. Engelman, F.D. Bushman, R. Craigie Identification of discrete functional domains...
First-principles calculation of the folding free energy of a three-helix bundle protein The folding and unfolding of a three-helix bundle protein were explored with molecular-dynamics simulations, cluster analysis, and weighted-histogram techn... E Boczko,C Brooks - 《Science》 被引量: 683发表:...