3a, b). To dissociate uptake from clearance, we performed a pulse-chase assay by preloading microglia with tau fibrils and assessing the time-dependent clearance in the next 24 h (Fig. 3c). Compared with corresponding littermate wild-type controls, inhibition of NF-κB slowed down, while ...
Pulse-chase experiment was performed for 48 h during RNAi to assay for CENP-A turnover (a). Quench-chase-pulse experiment was performed for the final 7 h of siRNA treatment to assay for CENP-A assembly (b). c, d shows typical image fields following the strategies in a, b, ...
The turnover rate of α-actinin assessed in a pulse-chase experiment.Xiaohu FanBryan G. HughesMohammad A. M. AliWoo Jung ChoWaleska LopezRichard Schulz
(D) The insertion of KgtP 275-AP was examined 40 min after IPTG addition by pulse-labeling. Lanes 1–2: cells transformed with pTRC-ftsY(WT); lanes 3–4: cells transformed with pTRC-ftsY(G385A). IPTG was added to the cells in lanes 2 and 4. Using this assay, we found that dis...
(C) A pulse–chase experiment demonstrates mRNA decay in the tethering assay. HeLa “Tet-off” cells were cotransfected with plasmids expressing β-6bs and βG from a tetracycline-responsive CMV promoter, as well as plasmids producing MS2 coat protein alone (cp, lanes 1–5) or fused to ...
We used an in situ 15N pulse-chase experiment in subtidal sands to follow the assimilation, trophic transfer, transformation, and flux pathways of MPB-N over 33 d. Throughout the study MPB dominated 15N uptake, on average representing only 18.1% of the biomass but 63.9% of the 15N within...
13CO2pulse-chase experiments monitored by high-resolution NMR spectroscopy and mass spectrometry can provide13C-isotopologue compositions in biosynthetic products. Experiments with a variety of plant species have documented that the isotopologue profiles generated with13CO2pulse-chase labeling are directly com...
A pulse-chase method to 13carbon-label douglas- fir seedlings for decomposition studies. Soil Sci.:46-53.Moore-Kucera J, Dick RP. A pulse-chase method to 13carbon-label Douglas-fir seedlings for decomposition studies. Soil Sci. 2008;173:46-53. https://doi.org/10.1097/ss.0b013e...
For pulse-chase assay, the cells were first incubated with pulse medium containing 2.5 × 108p.f.u. of phage for 24 h at 37 °C in a 5% CO2humidified atmosphere. The pulse medium was aspirated, and extracellular phages were removed by polysialic acid treatment procedure as describ...
Pulse-chase experiments (letting the ligand bind in the cold, then allowing it to internalize at 37 °C over time), were performed in MIO-M1 cells seeded on glass coverslips. Briefly, cells were serum-starved for 30 min and then incubated (pulse) on ice with pre-chilled solutions of...