The sections were incubated with anti-ZC3H13 (Bioss, P.R. China) and then with horseradish peroxidase (HRP)-labeled IgG secondary antibodies (Beyotime Institute of Biotechnology, Shanghai, P.R. China). Fields from each slide were examined and photographed using a light microscope (Olympus, ...
The resin blocks were sectioned at 60–80 nm steps using a Reichert Ultracut S ultramicrotome. The sections were mounted on copper Formvar/Carbon-coated grids, stained with 2% uranyl acetate followed by lead citrate (Reynolds, 1963), and viewed with a JEOL 1200EX microscope operating at 80...
Semi-thin (1 µm) sections were cut with a Leica EM UC6 Ultramicrotome and examined under a light microscope to establish proper orientation. Ultra-thin sections were cut with the same microtome and collected on pioloform-coated 1 hole slot grids (Ted Pella Inc, cat # 19244). ...
The CEN17 is targeted using a digoxigenin (DIG)-labeled probe and detected using an ultraView Red ISH DIG detection Kit. Under light microscopy, HER2 shows as discrete black signals, and chromosome 17 appears as red signals. The sections were loaded into the Ventana Benchmark XT machine. A ...
It is ensured by phase modulation of the illumination light at all wavelengths in our microscope (Figures S2 and S5). We examined the multicolor 3D imaging ability of the microscope by imaging a dual-color, labeled mouse mammary gland. The sample was first immunostained with Krt14/AF488, a ...
A normal surgical otomicroscope was used in the evaluation, and the findings were recorded via black and white and/or color photography; for recent cases... T Palva,H Ramsay - 《Otology & Neurotology》 被引量: 46发表: 2007年 The Violet Light Microscope: A Method for Visual Estimation of...
However, a major limitation in much of this work has been the inability to clearly visualize and then manipulate the target structures, many of which are either at or below the resolution of the light microscope. The combination of subcellular laser ablation with the use of gene fusion products...
Interestingly, CMAS was not observed on the suction side of the turbine blades (labeled as no. 3 and 4 in Fig. 4b). From this investigation it is clear that the CMAS formation and their infiltration on the turbine blade is greatly dependant on the exposed temperature. Fig. 4: Turbine ...
Methods First, we prepared a lateral flow assay test strip, and labeled the detection antibody using a fluorescent microsphere. Second, we optimized the antibody labeling conditions. Third, we optimized the detection conditions. Fourth, we created a working curve. Fifth, we conducted a methodological...
MSCs were fixed with 4% paraformaldehyde, stained using an iron staining kit (Sigma-Aldrich, USA), and visualized under a light microscope. In vitro assessment of cell-tissue matchmaking nanoconstruct (C-TMN) cytotoxicity We examined the possible toxic effects of C-TMN on rat MSC viability and...