Assessing genuine extracellular vesicle (EV) uptake is crucial for understanding the functional roles of EVs. This study measured the bona fide labelling of EVs utilising two commonly used fluorescent dyes, PKH26 and C5-maleimide-Alexa633. MCF7 EVs tagged with mEmerald-CD81 were isolated from cond...
In this work, gold nanoparticles coated with a fluorescently labelled (rhodamine B) silica shell are presented as fiducial markers for correlative light and electron microscopy (CLEM). The synthesis of the particles is optimized to obtain homogeneous, spherical core-shell particles of arbitrary size....
The integrated light microscope allows us to record the fluorescence signal while scanning the electron beam through the optical field of view. By correlating changes in the fluorescence decay with the instantaneous electron beam position and the other EM signals, we obtain a SR fluorescence image (...
Detection may be by conventional means, for example if the label is a fluorescent moiety, detection of an incorporated base may be carried out by using a confocal scanning microscope to scan the surface of the array with a laser, to image a fluorophore bound directly to the incorporated base...
electromagnetic radiation of a wavelength corresponding to an excitation wavelength of the fluorophore; and detecting fluorophore labelled biological components in the entire thickness of the measurement cavity, said detecting comprising acquiring a digital image of the irradiated area in the measurement ...
1 (right), overlaid to the phase contrast image, clearly shows the localization ofthe fluorescent dye inside the yeast shells that appear as pseudo-spherical particleswith a hydrodynamic diameter of ca. 6 渭m, as determined by dynamic light scatteringmeasurements.The same loading protocol was ...
The fluorescence imaging was performed with an inverted microscope (Olympus IX51, Tokyo, Japan) using an excitation filter BP460-490, beam splitter DM500 and a barrier filter BA520IF. The intensity of fluorescence emission was captured using a CCD DP71-1-5 camera (Olympus, Tokyo, Japan). ...
For microscopy, a Leica TCS SP5 II inverted confocal laser scanning microscope was used with HeNe laser (10 mW: 633 nm) as excitation light source: AOBS (Acousto-Optical Beam Splitter) (640–690 nm) and Leica HyD detector. Fluorophores were used at a final concentration of 2.5 µM and...
Inspection of the cleaned substrates with a portable optical microscope revealed detailed information into how physical properties of cleaning materials influence the location of residual soot on the surface. 3D digital light microscopy and Fourier-transform infrared spectroscopy were used ...
Interestingly, lesions with high radioactivities normally correspond to high expression of CD11b, expressed as integrated optic density (IOD) measured by Image Pro Plus, as described in the method section. Spearman correlation analysis clarified the relationship between the lesions' activity on SPECT/...