1.Transmission Electron Microscope (TEM) The transmission electron microscope is used to view thin specimens through which electrons can pass generating a projection image. The TEM is analogous in many ways to the conventional (compound) light microscope. TEM is used, among other things, to ima...
around the year 1590 (more history here). The compound microscope uses lenses and light to enlarge the image and is also called an optical or light microscope (versus an electron microscope). The simplest optical microscope is the magnifying glass and is good to about ten times (10x) magnific...
The fluorescence intensity can be measured by a fluorophotometer or a fluorescence microscope. Otherwise, individual cells or microorganisms are passed through an excitation light-irradiation region in a flow system at a high speed, the fluorescent light being measured and the data being treated stati...
Particle sizes and morphology of the radiolabeled particles were examined by light microscope, dynamic light scattering, and transmission electron microscope and found to be of stable spherical morphology within the range of 1.4-3.2 渭m. The preparation was injected into the knee joints of healthy ...
Cells grown on glass coverslips were transferred to an imaging chamber (Life Imaging Services, Switzerland) which was placed into an adapter on the microscope stage and was temperature-controlled by a Peltier device. The culture medium was changed to CO2-independent media (DMEM or DMEM/F12, Hepes...
The organs were dehydrate in 4% PFA with a graded sucrose series then embedded in OCT compound to obtain 5 µm cryo section. This was used to identify AD-MSCs with DiI staining. Statistical analysis Statistical analyses were performed using the by GraphPad Prism software (La Jolla, CA, USA...
p < 0.01. labeling of the radioactive compound outside of the realm of sites recognized by the un1abeled compound, if such nonselective binding were occurgrin . Indeed this was demonstrated by the inability of alprazolam to in hibit [3H]diazepam binding from the peripheral bind ing ...
The term “luminophore” is used herein to refer to an atom or atomic grouping in a chemical compound that manifests luminescence.The term “particle” or “microparticle” is meant to refer to small particles, preferred herein in diameter from about 0.01 micrometers to about 1000 micrometers,...
Results: Photohemolysis was dependent on excitation light intensity and fluorochrome concentration. Fluorescein isothiocyanate (FITC)-labeled BSA was the most phototoxic compound tested, inducing 50% of maximum response in 14min. The relative phototoxicity of the BSA conjugates was: FITC > BODIPYFL >...
In this study, l -arginine and phenylalanine amino acids labeled with stable isotopes 13 C and 15 N were localized in cultured cells with the ion microscope at 500nm spatial resolution. Cells were exposed to the labeled amino acids and cryogenically prepared. SIMS analyses were made in ...