CaLFY functions to maintain the undifferentiated status while MPL1 promotes differentiation through directly repressing CaLFY expression. During the process of LL initiation (P2 ~ P4 stages), CaLFY expression is high in the undifferentiated tip of the whole CLP but lower in the more ...
Washed the slides three times with PBS, and added horseradish peroxidase (HRP) labeled goat anti-rabbit secondary antibody for 30 min. The sections were developed using 3,3-diaminobenzidine tetrahydrochloride (DAB) and contrasted using hematoxylin. Images were acquired using a light microscope. For ...
Uorescence inverted microscope (Olympus, Tokyo, Japan). Nuclear/cytosolic fractionation. Subfractionation was carried out using a Nuclear/Cytosolic Fractionation Kit (Beyotime, Wuhan, China). IEC-6 cultures had been washed with ice-cold PBS, scraped through…...
N,N-dimethyltryptamine (DMT) is a component of the ayahuasca brew traditionally used for ritual and therapeutic purposes across several South American countries. Here, we have examined, in vitro and vivo, the potential neurogenic effect of DMT. Our resul
One of the most important properties of a confocal system is the point spread function (PSF) (see Supplement 1), which is mainly determined by the microscope objective. The resolution of CLSM can, therefore, be modifying the spatial arrangement of the excitation and detection PSFs [22,23], ...
(BCIP) in alkaline phosphatase solution containing 50 mmol/l MgCl2and 1 mmol/l levamisole was used to detect bound Dig-labeled Fab fragments. Images of the processed sections were collected by a Color view IIIu camera mounted on a BX61 microscope (both: Olympus), and Adobe Photoshop (Adobe...
N indicates the nucleous, open white arrows point to endoplasmatic reticulum, filled thick arrow to autophagic vacuole, asterisk for margination and condensation of chromatin and black circles show numerous vacuoles observed by electronic microscope. Full size image Cas III-ia induced death by ...
16, 17 These studies emphasized the importance of examining the overlap in functions amongst the multi-BH domain pro-apoptotic BCL-2 family members. The dramatic developmental abnormalities observed in Bax−/−Bak−/− DKO mice highlight the functional redundancy between BAX and BAK.17 ...
Plates were kept light-protected and imaged one hour later in a Perkin-Elmer Opera microscope using a 20x air objective (NA 0.4) and the following acquisition set: a 635 nm laser excitation line and a 690/50 emission detection filter for DRAQ5 detection. Five images were collected per well...
4-Iodo-2,5-dimethoxy-N-(2-methoxybenzyl)phenethylamine (25I-NBOMe) is a new psychoactive substance with strong hallucinogenic properties. Our previous data reported increased release of dopamine, serotonin, and glutamate after acute injections and a tole