Four behavioral tests were performed to evaluate the motor functions. All the mice underwent the four tests with an interval of 1 h between each test. 2.6.1. Rotarod test Mice were placed on the rotarod and then tested at a speed of 30 rpm for up to 120 s on the revolving rod. The...
Washed the slides three times with PBS, and added horseradish peroxidase (HRP) labeled goat anti-rabbit secondary antibody for 30 min. The sections were developed using 3,3-diaminobenzidine tetrahydrochloride (DAB) and contrasted using hematoxylin. Images were acquired using a light microscope. For ...
Transcripts of three of the four cricket opsins were detected in the compound eyes:in situprobes forgreenBlabeled the retina outside the DRA (Figure3A), probes foruvlabeled photoreceptors in all eye regions except for an area in the ventral half (Figure3B) and those forbluelabeled the DRA a...
N,N-dimethyltryptamine (DMT) is a component of the ayahuasca brew traditionally used for ritual and therapeutic purposes across several South American countries. Here, we have examined, in vitro and vivo, the potential neurogenic effect of DMT. Our resul
CaLFY functions to maintain the undifferentiated status while MPL1 promotes differentiation through directly repressing CaLFY expression. During the process of LL initiation (P2 ~ P4 stages), CaLFY expression is high in the undifferentiated tip of the whole CLP but lower in the more ...
N indicates the nucleous, open white arrows point to endoplasmatic reticulum, filled thick arrow to autophagic vacuole, asterisk for margination and condensation of chromatin and black circles show numerous vacuoles observed by electronic microscope. Full size image Cas III-ia induced death by ...
After RNA isolation for each sample, double stranded cDNA was synthesized from 500 ng total RNA and biotin-labeled using the GeneChip 3' IVT Express Kit (Affymetrix, Santa Clara, CA, USA, #901228-A) per the manufacturer's instructions and protocol. RNA was purified, quantified, fragmented ran...
The laser block is composed by: an active region (light orange bar in the picture), with an anti-reflection coated facet (labeled as 2); an external Littrow grating (multi-colored milled bar) placed at distance L from the facet 2; a coupling mirror (grayscale bar near the Littrow ...
. A Zeiss ELYRA S1 SR-SIM microscope was used with UV light and solid-state laser (405/488/561/642 nm) illumination sources, a 100× oil immersion objective (NA = 1.46), and a sCMOS camera. Typical imaging was performed on a 49.34 × 49.34 μm2 area with 1280 ...
The slides are imaged with 10× and 20× objectives using an Openlab™ -controlled microscope. 3. Annexin Assay The present invention may also employ the use of the Annexin V assay in examining the effects of BMS-34551 or an analog thereof on melanoma cancer cells. An early process in...