Since the development of the first microscope by the pioneer Zacharias Jansen (16th century), Robert Hooke (17th century) and later by Anton van Leeuwenhoek (18th century), it has become evident that biological entities have optical properties. The field of imaging methodologies now focuses on vis...
Collectively, our work provides a comprehensive resource for decoding the aging process of microglia, shedding light on how microglia maintain brain functions.Similar content being viewed by others Age-associated microglial transcriptome leads to diminished immunogenicity and dysregulation of MCT4 and P2RY...
Adult fireflies exhibit unique flashing courtship signals, emitted by specialized light organs, which develop mostly independently from larval light organs during the pupal stage. The mechanisms of adult light organ development have not been thoroughly studied until now. Here we show that key homeobox ...
Atomic Force Microscope SEM: Scanning Electron Microscope TEM: Transmission Electron Microscope DLS: Dynamic light Scattering References Mirkin MV, Sun T, Yu Y et al (2016) Electrochemistry at one nanoparticle. Accounts Chem Res 49:2328–2335 Google Scholar Bard AJ, Zhou H, Kwon SJ (2010...
By irrigating plants using water labeled with deuterium, xylem velocity and dynamic water distribution can be measured. Information on xylem flow can also be obtained with sap flow meters that are placed around stems and which function using a variety of heat pulse-based methods. Non-invasive ...
The prognosis of diffuse large B-cell lymphoma (DLBCL) is heterogeneous. Therefore, we aimed to highlight predictive biomarkers. First, artificial intelligence was applied into a discovery series of gene expression of 414 patients (GSE10846). A dimension
Fig. 7. Fluorescence microscope images of HEK-293 cells labeled with 50 μM GFZnP ED (2) (A) before and (B) after Zn2+ addition, followed by (C) Zn2+ withdrawal. (D) Mean fluorescence intensity as a function of time. Since GFZnP ED (2) is completely water soluble, for 2PM,...
On the contrary, this compound caused reduction of the levels of the two mRNAs and counteracted the effect of the cAMP analogue. These findings could indicate that PKA positively modulates mRNA levels of the sGC subunits in these cells and similarly explain the observed biphasic effect of Rp-8...
To visualize the photoprotein, embryos were acclimatized to the dark and imaged under a GFP filter set on a Zeiss compound microscope (Additional file8). The blue fluorescent light excites the photoprotein in these cells causing them to emit light at around 485 nm to 496 nm[16, 27]. In...
Wide-field fluorescence imaging was performed using a NIKON E800 compound microscope equipped with a SPOT RT-slider digital camera (Diagnostic Instruments Inc.) interfaced to a computer. GFP excitation was performed with standard FITC filters. Images of the roots were taken through FITC filters under...