Whereas fluorescence is not directly visible in a standard electron microscope, gold particles, as the most common choice of probe for EM can also only be visualized using specialized light microscopes. In this review we will discuss some of the latest developments of probes for CLEM and some ...
For fluorescence imaging, these ultrathin tissue sections were directly examined with a cLSM. The ultrathin sections were stained with toludine blue and observed under a light microscope (Leica-DMIL, Germany). Results and discussion Trioctylphosphine oxide capped CdSe QDs were synthesised and made ...
Light microscope radioautography was used to visualize ascending serotonin (5-HT) systems in adult rat brain, following prolonged latero-ventricular instil... A.,Parent,and,... - 《Neuroscience》 被引量: 710发表: 1981年 Intraventricular administration of BDNF increases neuropeptide expression in new...
Multiview light-sheet microscope for rapid in toto imaging. Nat. Methods 9, 730–733 (2012). CAS PubMed Google Scholar Wu, Y. et al. Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy. Nat. Biotechnol. 31, 1032–1038 (2013). CAS PubMed PubMed ...
The excitation light was focused on the back focal plane of a 100 Â oil objective (PLAPO 100 Â , total internal reflection fluorescence mode, numerical aperture 1.45, Olympus) mounted on an inverted microscope (Olympus IX71). The emission was recorded using an electron multiplying charge-...
Sections from all mice were imaged using the same microscope and camera settings. For each of these sec- tions, we measured the area with labelled cells. By mul- tiplying the sum of areas from a series by 320 μm, we obtained an estimate of the volume of cerebellum con- taining ...
At the outset of the session, part of the slide with no tissue (blankfield) was viewed in the microscope and a white balance carried out. The level of illumination was monitored as being between 204 and 206 for the blankfield (between photographs the illumination level was checked as being...
We labelled the Pil1-CLIP expressing cells with the substrate Atto565-CLIP and imaged the cells with a custom built STED-microscope [26]. Using STED microscopy, due to the improved optical resolution, we could reveal details that were concealed in the diffraction limited ...
For subcellular localization experiments, cells were imaged using LSM 880 (Zeiss, Oberkochen, Germany) confocal laser scanning microscope, using a 488 Argon laser with a 490–553 nm emission filter for GFP; Alexa 647 and DAPI labelling were acquired with Diode Pumped Solid State Lasers (DPSS)...
Widefield microscopy images were acquired for experiments using the chimeric IL-1 constructs using a Zeiss Axioimager.D2 upright microscope using a ×20/0.5 EC Plan-neofluar objective and captured using a Coolsnap HQ2 camera (Photometrics) through Micromanager software v1.4.23. Specific band pass...