The ability to monitor micropipette injections with a high‐resolution fluorescent microscope has utility for a variety of applications. Herein, different approaches were tested for creating broad‐band fluorescently labelled glass micropipettes including: UV cured glass glues, baked glass enamel containing...
Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonab... K Elliott,DS Hill,C Lambert,... - 《...
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Interleukin-1α is a suggested dual-function cytokine that diverged from interleukin-1β in mammals potentially by acquiring additional biological roles that relate to highly conserved regions in the pro-domain of interleukin-1α, including a nuclear localisation sequence and histone acetyltransferase-bind...
DNA nanotechnology is an emerging field that promises fascinating opportunities for the manipulation and imaging of proteins on a cell surface. The key to progress is the ability to create a nucleic acid–protein junction in the context of living cells. Here we report a covalent labelling reaction...
Double-labelling immunohistochemistry for MGMT and a cocktail of non-tumourous elements provides a "one look" method for determining whether tumour cell nuclei are MGMT-positive or MGMT-negative. This can be used to infer the methylation status of the tumour. There is good observer agreement and...
branches to the soleus and gastrocnemius muscles;3.in 13 rats,one tracer was injected into the tibial nerve and the other into the bladder wall.The sections of the L_(3-6),S_(1-3) dorsal root ganglion were stu- died with a olympus fluorescence microscope using UG-1 excitation filter ...
During the experiment images were acquired at a rate of 1 Hz using a Nikon Eclipse TE2000-S microscope equipped with a Nikon CFI Plan Apo VC 60× Oil objective 1.4 (NA) and a CCD camera (iXonEM + DU885, Andor Technology). A 479 nm monochromator was used for excitation and the ...
For subcellular localization experiments, cells were imaged using LSM 880 (Zeiss, Oberkochen, Germany) confocal laser scanning microscope, using a 488 Argon laser with a 490–553 nm emission filter for GFP; Alexa 647 and DAPI labelling were acquired with Diode Pumped Solid State Lasers (DPSS)...
The nanoSIMS technol- ogy analyses the stable- or radioactive-isotope content of single cells at a resolution (50 nm) that exceeds a Raman microscope, possesses sensitivity in cellular 14C detection that exceeds MAR by 1000 Â , has a stable-isotope measurement preci- sion of 71%, and a...