After a 24‐h incubation, the migrated cells on the bottom side of the top filter were directly observed with a fluorescence microscope (Keyence). The migrated cells were analyzed with Hybrid Cell Count software (Keyence). Results were presented as a rate of the occupied area by migrated ...
UV-vis spectrometry for cell samples was measured on a Synergy H4 microplate reader (Bio-Tek) with Gen5 software (v3.11.19, BioTek). Confocal microscopy images were obtained on an LSM 700 laser scanning confocal microscope (Zeiss) with ZEN software (v2.1, ZEISS). Flow cytometry was ...
You have a need for sophisticated, highly specialized and unique lenses? You need small adjustments to existing lenses? Leica Microsystems HC System The Leica Microsystems HC system (Harmonic Compound System) comprises the optical components that have been matched to each other for optimal image g...
In an implementation, the labeling of the compounds of essential oils may comprise using a fluorescent dye that covalently links the hydroxyl group to the fluorescent dye, such that the compound may be detected using a fluorescence microscope.Joshua James Plant...
If the F-actin is depolymerized, phalloidin will not bind. Any processing steps or any treatments/compound that can promote depolymerization would impact phalloidin binding. Also, any compounds that also bind to F-actin (without depolymerization) may affect phalloidin binding. ...
Upright compound microscope with 40x water immersion objective Leica Microsystems DM6000B MicroPoint Manual Laser Illumination System Photonic Instruments Phenylthiourea (PTU) Alfa Aesar L06690 Tricaine Sigma-Aldrich E10521 Proteinase K Roche Group 03115836991 Plastic thread Any Supplier Agarose ...
在本研究中,Cur在一分钟内从5倍家族性阿尔茨海默氏病(5xFAD)的遗传小鼠模型和人类AD组织中,用组织化学方式标记A+斑块。Cur 的标签能力与传统的淀粉样结合染料(如硫黄素-S(硫黄素-S)、刚果红 (CR) 和氟玉 C (FJC) 以及 A+ 特异性抗体 (6E10 和 A11)进行了比较。我们观察到,与这些传统染料相比...
One-bead one-compound (OBOC) libraries produced by combinatorial synthesis are still the most efficient and most flex- ible method to generate a large number of substances. If the molecules produced by OBOC chemistry could be screened against targets directly at the site of synthesis and if...
After fixation and immunocytochemistry labeling, neurons were imaged on a confocal scanning microscope. b Colocalization analysis of SiR-tz and FLAG intensity in the region within the dashed box from panel a, with Pearson’s correlation coefficient (PCC) shown above the scatterplot. Fluorescence ...
(Carl Zeiss, Inc.) microscope with a 60× oil immersion objective. The cell was stained with MTG and Coupa for 375 s using a laser intensity of 100% at 488 nm for MTG and 405 nm for Coupa. A recovery image was obtained at a 15-s interval. The fluorescence intensity of the ...