After a 24‐h incubation, the migrated cells on the bottom side of the top filter were directly observed with a fluorescence microscope (Keyence). The migrated cells were analyzed with Hybrid Cell Count software (Keyence). Results were presented as a rate of the occupied area by migrated ...
2.Protect the overlap position of the different polarities of nickel strips, pads, traces and plugs and leads on the BMS board: insulation is required to completely cover the contact surface that can cause a short circuit between the t...
Briefly, prostate cancer tissue specimens were immediately embedded in optimal cutting temperature (OCT) compound and snap-frozen (as tissue block) in liquid nitrogen [36]. Frozen tissue blocks were stored at −80 °C until further processing. The Sects. (4–5 µm) were cut using a ...
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible Cy5 filter set Quantity1 Ea. Product LineAlexa Fluor™ Shipping ConditionQualified for Ambient or Wet Ice Label TypeAlexa Fluor Dyes Product TypePhalloidin ...
UV-vis spectrometry for cell samples was measured on a Synergy H4 microplate reader (Bio-Tek) with Gen5 software (v3.11.19, BioTek). Confocal microscopy images were obtained on an LSM 700 laser scanning confocal microscope (Zeiss) with ZEN software (v2.1, ZEISS). Flow cytometry was ...
In an implementation, the labeling of the compounds of essential oils may comprise using a fluorescent dye that covalently links the hydroxyl group to the fluorescent dye, such that the compound may be detected using a fluorescence microscope.Joshua James Plant...
Finally, cells were transferred to a SP8 Leica spectral confocal microscope equipped with a temperature control chamber (Okolab) for time-lapse live image acquisition. Images were acquired every 30 s for 10–20 min at 37 °C using the 488 nm, 561 nm and 633 nm lasers...
Proximity labeling with a membrane-impermeant biotin-phenol compound restricted labeling to the cell surface, and Label-Free Quantitation (LFQ) mass spectrometry combined with ratiometric HRP tagging of membrane vs. synaptic surface proteins was used to identify the proteomic content of excitatory clefts...
Incubate the cells in a 37°C, 5% CO2incubator for 30 minutes to 2 hours. Wash the cells with HHBS (Component B), and add growth medium or HHBS back to the cells. Image the cells using a fluorescence microscope with DAPI filter (Ex/Em = 360/445 nm). ...
Imaging of discs was conducted on the following microscopes: Leica SP5 Confocal Microscope, and Zeiss LSM 800 Confocal Microscope with AiryScan. All DNA labeling images shown in the main figures were acquired on the Zeiss LSM 800 with AiryScan processing. All image visualization and processing was ...