Proven performance—over 10 years of clinical real-time PCR instrument manufacturing Designed with the clinic in mind—interactive diagnostic instrument with short run times, minimal maintenance, and use of existing plastic consumables Enhanced security—auditing a...
Proven performance—over 10 years of clinical real-time PCR instrument manufacturing Designed with the clinic in mind—interactive diagnostic instrument with short run times, minimal maintenance, and use of existing plastic consumables Enhanced security—auditing a...
注册资本:50万 人民币 企业性质:私营独资企业 主营产品:生化试剂,标准品,对照品,PCR试剂盒, 核酸检测试剂盒,荧光定.. 公司地址:上海闵行区莘福路388弄2号楼 进入商铺 收藏商铺 #技术#PCR检测试剂盒试验的循环参数 #动态#ELISA试剂盒试验处理保存方法产品...
USA) using Lipofectamine 3000 Reagent (Life Technologies, USA) according to the manufacturer’s protocol.PLOD2 knockdown was validated by quantitative RealTime PCR and by Western blotting 24 and 48 h after transfection, respectively
each channel with 8 bytes read/write buffer Supports ADC Data Ready and DAC Data Loaded indication Supports periodic data acquisition Supports late sample for high latency device Supports external interrupt input SPI Slave Interface Supports Mode 3 timing modes Supports MSB first transfer fashion Local...
The PCR reaction was performed for 15 cycles, in 96-well plates, using the Phusion high-fidelity polymerase with HF buffer (Thermo Fisher). Following the PCR reaction, 1 µL of DpnI (NEB) was added to each well, along with Cutsmart buffer (NEB), and incubated at 37 °C for 45...
The primer sequences for RT-PCR were listed in Table S1. Statistical analysis According to our preliminary experiments, for Wnt5a analysis, group sample sizes of 17 and 34 achieve 85% power to reject the null hypothesis of equal means when the population mean difference (effect size) is μ...
1.本产品能与各种PCR酶配合使用,能够扩增具有复杂二级结构模板(GCrich、重复序列等) 的PCR片段。 2.使用GCBuffer进行PCR扩增时,建议使用Tm值较高的引物。因此,引物最好设计在30 mer左右。 3.使用GCBuffer扩增的GCrich的DNA片段长度,会因GC含量不同而各有差异。 4.由于GCBuffer和一般的PCRBuffer相比DNA变性效果较...
B: Real-time PCR analysis of hepatic Grp78 mRNA amounts in RD-fed and HFD-fed mice (n = 3). C: Immunoblot showing effects of THA (500 nM) treatment on HDAC5 phosphorylation in primary hepatocytes at indicated times. D: Immunoblot showing effect of THA (500 nM, 1 h) treatment on ...
Quantitative real-time PCR (qRT–PCR) was used to detect levels of HCG18, miR-29a/b, and mRNAs. MTT, colony formation, wound healing and Transwell assays were used to evaluate cell proliferation, migration and invasion, respectively. A luciferase reporter assay was utilized to evaluate NF-κB...