PCR purificationgel extractionkitsBACKGROUND Both polymerase chain reaction (PCR) purification kits and DNA gel extraction kits have been widely used for DNA purification in laboratories worldwide. However, the uses of these kits are costly and produce laboratory wastes that have negative impacts on ...
Among other things, the compositions, methods, and kits are suitable for one-step lysis and amplification of nucleic acid sequences of interest. In general, the compositions comprise TCEP and a non-ionic surfactant, such as Triton X-100....
Visit ChemicalBook To find more PCR BUFFER information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. You can also browse global suppliers
就是一些缓冲液，用来保障pcr扩增的进行。
品牌:Qiagen 所属分类:工具酶 货品编号: qiagen.19066 品牌: qiagen 品名: Buffer PB 规格: 500ml Buffer PB is used in DNA cleanup procedures and enables efficient binding of single- or double-stranded PCR products to the spin-column membrane. Buffer QG is a solubilization and binding buffer (with...
The 10x PCR buffer without MgCl2 has been used with PCR enzyme. The 10X PCR buffer without MgCl2 has been used as a component in the PCR amplification mixture for the amplification of mycotoxin mold DNA, parasite DNA and Fusarium oxysporum DNA. Product specifications and parameters: SKU Name Sp...
Table 2. Common additives or co-solvents used as PCR enhancers, and their recommended final ...
Sign In PCR (polymerase chain reaction) is a laboratory technique used to amplify DNA (deoxyribonucleic acid) segments. Our ready to use PCR buffer contains several components essential for the PCR reaction. The PCR reaction buffer helps to maintain a stable pH during the PCR reaction. It also...
Robust PCR amplification of GC-rich human genomic DNA A serial dilution in duplicate of human genomic DNA (1 μg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng, 1-6 respectively) was used with primers and Taq DNA Polymerase to amplify a 450 bp fragment of the human myc gene. The ...
aBased on the instruction of manufacturer, the PCR reaction (25 μL) used 0.125 μL of Taq polymerase (1.25 U), 0.5 μL of primers (5 pmol), 1 μL of 10-fold diluted DNA template (approximately 1 ng), 2.5 μL of 10-fold PCR buffer, 1.5 μL of MgCl2 (50 mM ), and lastly...