主营产品:生化试剂,标准品,对照品,PCR试剂盒, 核酸检测试剂盒,荧光定.. 公司地址:上海闵行区莘福路388弄2号楼 进入商铺收藏商铺 #动态#培养基的制备步骤 用途:仅供科研实验 产地:国产 品牌:派瑞曼 包装规格:50μg、200μg、1mg 货号:P-Db1683
Restorase functions by modifying the damaged sites allowing subsequent template copying. Restorase can successfully produce PCR products from 0.2 to 20 kb in length.The level of template damage will dictate optimal Restorase treatment of the DNA. Lightly damaged DNA will require a shorter ...
PCR 反应 (25 超微量 (µ L) 根据制造商的指示,使用 0.125 超微量 (µ L Taq 聚合酶 (1.25 U)、 0.5 超微量 (µ L 底漆 (5 pmol),1 超微量 (µ L 10 倍稀释 DNA 模板的 (大约 1 吴)、 2.5 超微量 (µ L 10 倍聚合酶链反应的缓冲,1.5 超微量 (µ L MgCl2 的 (50 毫米),和...
主营产品:DNA提取试剂盒,RNA提取试剂盒,蛋白提取试剂盒,PCR试剂,动物红细.. 公司地址:紫雀路33号院3号楼3516 进入商铺收藏商铺 CAS编号:9024-52-6 产地:北京 品牌:百奥莱博 货号:L10337 英文名称:Aldolase from rabbit muscle(Suspension) 保质期:长期个月 ...
A day after transfection, cells were fixed with 3.7% paraformaldehyde for 15 min at room temperature and washed three times with PBS. Subsequently, non-specific binding sites were blocked with 3% non-fat dry milk in 50 mM Tris-PBS pH 7.4 (blocking buffer) for 1 h at room ...
The HuP10 cDNA clone (8069212) was purchased from Open Biosystems, Dharmacon, Lafayette, CO, USA. It was used as a template to amplify the gene by PCR using a forward (5′-CGCGGATCCATGTTCCCACTGACTGAGGAAAACAAGC-3′) and a reverse (5′-CGCCTCGAGTTACTAGTCATCCAGAGCAGGTGG-3′) primer...
细胞过表达cyclin D1, Bcl-2, c-Myc 及 Rb 蛋白。 Bcl-1/J(H)基因重排得到了PCR证实。 JeKo-1细胞在SCID小鼠中高成瘤。 [PubMed: 9753063];细胞传代方法:1:2传代。3天内可长满。;细胞生长特性:悬浮生长;细胞形态特性:淋巴母细胞样;相关细胞有:H-520细胞、NCIH1975细胞、33604细胞 ...
PCR: Polymerase chain reaction PD: Protein D PHiD-CV: Pneumococcal-Haemophilus influenzae protein D conjugate vaccine POET study: Pneumococcal Otitis Efficacy Trial QoL: Quality of life RCT: Randomised controlled trial RR: Relative risk SAE: Serious adverse event VE: Vaccine efficacy WH...
In both NILs, PCR results identified a strong bias towards Ler DNA at markers MT435 (~32 cM) and nga168 (~73 cM) on chromosome 2 and ciw9 (~90 cM; zll-1) on chromosome 5, while the rest of the genome was biased towards Col. Subsequent mapping of all individual phenotypic NIL22 ...
Real-time PCR Total RNA was purified using TRIzol reagent (Invitrogen). cDNA synthesis was performed using SuperScript II Reverse Transcriptase (Invitrogen) after DNase I treatment (Invitrogen), and real-time PCR was then run using Thunderbird SYBR Green qPCR mix (Toyobo) and results were analysed...