英文名称:ReadiUse PCR Reaction Buffer 运输:低温运输 产地:西安瑞禧生物 描述:ReadiUse PCR 反应缓冲液是一种预配好的高效PCR反应缓冲体系,适用于常规PCR、RT-PCR以及各种DNA扩增应用。该产品具有优化的pH值、离子强度和稳定成分,有助于提高DNA聚合酶的活性、扩增效率以及反应的特异性和产量。该缓冲液为即用...
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
英文名称:PCR Reaction Buffer, Taq Reaction Buffer complete - 10 x总访问:974 国产/进口:进口半年访问:31 产地/品牌:德国Jena产品类别:生化试剂 规格:PCR-262-c最后更新:2024-12-5 货号: CAS 号: 参考报价:2373 立即询价电话咨询 [发表评论][本类其他产品][本类其他供应商][收藏] ...
PCR reaction Purification bufferThe present disclosure relates to buffers containing polyols for use with affinity-binding and/or magnetically susceptible thermoplastic particles and methods of making and use thereof.レスリー,トーマス マークポン,ジンリン...
10× PCR Buffer Product Summary DNase, RNase: None detected Suitable for use in the Polymerase Chain Reaction (PCR) when diluted to 1× concentration (see comments section).Comments Information about licenses to PCR can be obtained from The Perkin-Elmer Corporation or Roche Molecular Systems,Inc....
Nested PCR - use to synthesize more reliable product - PCR using a outer set of primers and the product of this PCR is used for further PCR reaction using an inner set of primers. Inverse PCR- for amplification of regions flanking a known sequence. DNA is digested, the desired fragment ...
Consider the standard PCR reaction mix (25 µL reaction) below. All reactions are run for 30 cycles. Table 1.PCR reaction components COMPONENTVOLUMEFINAL CONCENTRATION1. autoclaved ultra-filtered water (pH 7.0)20.7µL-2. 10x PCR Buffer*2.5µL1x3. dNTPs mix (25 mM each nucleotide)0....
We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage....
Key components of a PCR amplification reaction are a thermostable DNA polymerase, Deoxynucleotide Triphosphates (dNTPs), reaction buffer and magnesium, two oligonucleotide primers and a thermal cycler that allows controlled cycling of the reaction temperature through multiple rounds of DNA denaturation, pri...
Some scientists prefer to use reaction buffers that already contain MgCl2 at a final concentration of 1.5mM. It should be noted, however, that Hu et al. (1992) reported performance variability of reaction buffer solutions containing magnesium. The free magnesium changes of 0.6mM observed in their...