Here, we repurposed and integrated multi-RNA-sequencing (RNA-seq) analyses of lncRNA expression profiles among entries in the breast tissue cohort from The Cancer Genome Atlas (TCGA) database to identify the lncRNAs that are enriched in both breast cancer (cancer versus normal) and ER-negative...
Cells were grown photoautotrophically at 250 μmol photons m− 2 s− 1 under continuous bubbling with air in the TBP growth medium. Cells were harvested by centrifugation at 1000g for 3 min at 4 °C. Samples were resuspended with ice-cold sonication buffer containing 50 mM Tricine (pH...
The bone marrow cells were flushed from the medullary cavities of femurs and red blood cells were lysed using RBC lysis buffer (Yeasen, China). Next, the bone marrow cells were cultured in RPMI 1640 medium with 10% FBS containing 20 ng/mL recombinant murine M-CSF (Peprotech) and 1% P/...
Recently, utilization of Machine Learning (ML) has led to astonishing progress in computational protein design, bringing into reach the targeted engineering of proteins for industrial and biomedical applications. However, the design of proteins for emerg
Cell extracts were prepared 36 hours after transfection in passive lysis buffer, and the reporter activity was measured using the Dual Luciferase Reporter Assay System (Promega, Madison, WI). Wound-Healing Migration Assay Two-dimensional migration assay was performed using a scratch/wound model. ...
Finally, the pellet containing liver mitochondria was washed twice by suspension in the isolation buffer and centrifuged at 8700×g for 10 min. The protein concentration of mitochondrial suspensions was determined using the Biuret method with bovine serum albumin as a standard. Liver mitochondrial ...
The RNA 3X SHAPE buffer (333 mM HEPES, pH 8.0, 20 mM MgCl2, 333 mM NaCl) was added and the RNA was allowed to equilibrate at 37 °C for 10 min. Then, injecting the folded RNA probe to each stage (egg, 1-cell, 4-cell, 64-cell, sphere, shield) of zebrafish embryos...
EcR-B1 or anti-USP1 was diluted 1:2000 in 2% non-fat milk in TBS (10 mM Tris-HCl, pH7.5; 150 mM NaCl), and the second antibody of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG or HRP-was conjugated goat anti-mouse IgG diluted 1:10,000 in the same blocking buffer....
Quantitative real-time PCR RNA was isolated from cellular samples using the RNeasy Mini Kit (Qiagen) as described by the manufacturer. Cells were collected by centrifugation, washed twice in ice-cold PBS, and resuspended in Buffer RLT containing 40 µM DTT. The resulting mRNA yield was det...
Postoperative cognitive dysfunction (POCD) affects the outcome of millions of patients each year. Aging is a risk factor for POCD. Here, we showed that surgery induced learning and memory dysfunction in adult mice. Transplantation of feces from surgery m