The structure of the CoA is similar but less stretched than that of the CoA-moiety of the covalent enzyme-CoA-thioester in 4-hydroxybutyrate CoA-transferase from Shewanella oneidensis. In contrast to the structures of the apo-enzyme and enzyme-CoA-thioester, the structure described here has a ...
eutropha and 4-hydroxybutyrate CoA-transferase (OrfZ) from C. kluyveri into E. coli would result in the establishment of a new metabolic pathway, which competes with several existing pathways leading to citrate and acetate formation and to fatty acid synthesis (Figure 5). When the available ...
kluyveri; 4hbD , 4-hydroxybutyrate dehydrogenase; orfZ, 4HB-CoA:CoA transferase; phaC, PHA synthase; sad and gabD, succinate semialdehyde dehydrogenase of E. coli. Full size image E. coli possesses two forms of SSA dehydrogenase (SSADH) encoded by sad and gabD first identified in R. ...
Define 4-hydroxybutyrate. 4-hydroxybutyrate synonyms, 4-hydroxybutyrate pronunciation, 4-hydroxybutyrate translation, English dictionary definition of 4-hydroxybutyrate. n. 1. The third letter of the Greek alphabet. See Table at alphabet. 2. The third it
In this study, a propionate CoA-transferase (H16_A2718; EC 2.8.3.1) from Ralstonia eutropha H16 (Pct Re ) was characterized ... Elena,Volodina,Marc,... - 《Applied Microbiology & Biotechnology》 被引量: 5发表: 2014年 Targeted quantitative profiling of metabolites and gene transcripts associ...
1. The enzymes beta-ketothiolase, acetoacetyl-CoA reductase, acetoacetate-succinate CoA-transferase (;thiophorase') and d(-)-3-hydroxybutyrate dehydrogenase have been partially purified from crude extracts of glucose-grown nitrogen-fixin... PJ Senior,EA Dawes - 《Biochemical Journal》 被引量: 517...
Crystal structure of the complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoAdoi:10.1007/s00203-011-0737-2Sofia MacieiraJin ZhangWolfgang BuckelAlbrecht MesserschmidtSPRINGERMacieira, S., Zhang, J., Buckel, W., and Messerschmidt, A. (2012) Crystal structure of ...
Abstract To provide 4‐hydroxybutyryl‐CoA for poly(3‐hydroxybutyrate ‐co‐ 4‐hydroxybutyrate) formation from glutamate in Escherichia coli, an acetyl‐CoA:4‐hydroxybutyrate CoA transferase from Clostridium kluyveri, a 4‐hydroxybutyrate dehydrogenase from Ralstonia eutropha, a γ‐aminobutyrate:2‐...
To provide 4‐hydroxybutyryl‐CoA for poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) formation from glutamate in Escherichia coli, an acetyl‐CoA:4‐hydroxybutyrate CoA transferase from Clostridium kluyveri, a 4‐hydroxybutyrate dehydrogenase from Ralstonia eutropha, a γ‐aminobutyrate:2‐ketogl...
(3HP-co-4HB), was synthesized by Escherichia coli harboring a synthetic pathway consisting of five heterologous genes including orfZ encoding 4-hydroxybutyrate-coenzyme A transferase from Clostridium kluyveri, pcs鈥 encoding the ACS domain of tri-functional propionyl-CoA ligase (PCS) from ...