2-Hydroxyisobutyryl-CoA MutaseMTBE degradationHeterologous proteinsCo-expressionA genetic Fluorescence Resonance Energy Transfer (FRET) detector undergoes a post-translational modification (PTM)-induced conformational change that results in increased FRET. To test if the PTM-dependent FRET change can be ...
Hence, these enzymes play key roles in the degradation of acetone and define an involvement of CoA esters in the pathway. Further, the involvement of 2‐hydroxyisobutyryl‐CoA strongly indicates that the carbonyl‐C 2 of acetone is added, most likely, to formyl‐CoA through a TDP‐dependent ...
2-hydroxyisobutyryl-CoA mutaseMethyl tert-butyletherAquincola tertiaricarbonis L108DegradationA genetic Fluorescence Resonance Energy Transfer (FRET) detector undergoes a post-translational modification (PTM)-induced conformational change that results in increased FRET. To test if the PTM-dependent FRET ...
Kurteva-Yaneva N, Zahn M, Weichler MT, Starke R, Harms H, Muller RH, Strater N, Rohwerder T. 2015. Structural basis of the stereospecific- ity of bacterial B12-dependent 2-hydroxyisobutyryl-CoA mutase. J Biol Chem 290:9727-9737. http://dx.doi.org/10.1074/jbc.M115.645689....
Mechanistic details of the actinobacterial lyase-catalyzed degradation reaction of 2-hydroxyisobutyryl-CoAdoi:10.1016/j.jbc.2021.101522Michael ZahnGerhard KoenigHuy Viet Cuong PhamThore Rohwerder