An example is 4-hydroxybutyryl-CoA dehydratase (4-BUDH) from Clostridium aminobutyricum that interconverts 4-hydroxybutyryl-CoA to crotonyl-CoA. 4-BUDH is functional as a homotetramer with two active sites per dimer and displays a distinctive active site architecture with the [4Fe–4S] 2+ ...
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Scherf U, Buckel W (1993) Purification and properties of an iron-sulfur and FAD-containing 4- hydroxybutyryl-CoA dehy- dratase/vinylacetyl-CoA delta 3-delta 2- isomerase from Clos- tridium aminobutyricum. Eur J Biochem 215:421-429
Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds, and methods related thereto The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or ...
3-Hydroxybutyryl-CoA dehydrogenase is an enzyme that catalyzes the second step in the biosynthesis of n-butanol from acetyl-CoA, in which acetoacetyl-CoA is reduced to 3-hydroxybutyryl-CoA. To understand the molecular mechanisms of n-butanol biosynthesis, we determined the crystal structure of 3-...
s have been synthesised from (R)- and (S)-glycidol, respectively, and used to demonstrate that it is the pro-(S) hydrogen atom that is stereospecifically abstracted from C-3 of 4-hydroxybutyryl-CoA by 4-hydroxybutyryl-CoA dehydratase, and that this atom is not returned to C-4....
(1996) 4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron- sulfur clusters involved in an overall non-redox reaction, Biochemistry. 35, 11710- 11718.Muh, U., Cinkaya, I., Albracht, S.P., and Buckel, W. (1996) 4-Hydroxybutyryl-CoA dehydratase ...
The Nitrosopumilus maritimus 4-hydroxybutyryl-CoA synthetase (ADP-forming; Nmar_0206) represents one of several enzymes from this cycle that exhibit increased efficiency over crenarchaeal counterparts. This enzyme reduces energy requirements on the cell, reflecting thaumarchaeal success in adapting to low...
Willadsen P, Buckel W. Assay of 4-hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum. FEMS Microbiol Lett. 1990 Jul; 58 (2):187–191.Willadsen P, Buckel W. Assay of 4-hydroxybutyryl-CoA dehydratase from Clostridium ami- nobutyricum. FEMS Microbiol Lett 1990;70:187-92....
4-Hydroxybutyryl-CoA dehydratase from Clostridium amihobutyricum: Studies on the reaction mechanism and proposed role of the cofactors1. During fermentation with whole cells of Acidaminococcus fermentans or Clostridium microsporum the pro-3S hydrogen of (R)-2-hydroxyglutarate or of its precursor (S)-...