用无菌的0.01mmol/L PBS(PH7.2)收集幽门螺杆菌,使用核酸蛋白仪测菌液浓度,在实验前将其调整至1×109CFU/mL备用。 1.2.2 AGS细胞培养:将取出细胞悬液的冻存管迅速放在37℃水浴中轻轻摇晃解冻,然后加入4mL培养基混合均匀。1000RPM离心4分钟后弃上清,再加1-2mL培养基后轻轻抽吸均匀。将所得细胞悬液加入培养瓶中...
Although regulation of the inflammasome is related to crucial inflammatory disorders such as sepsis, pharmacological inhibitors that effectively inhibit inflammasome activity are limited. Here, we evaluated the effects of a phospholipase D1 (PLD1)-selective inhibitor (VU0155069) against sepsis and ...
CFU: colony forming unit CRISPR: clustered regularly interspaced short spaced palindromic repeats DNA: deoxyribonucleic acid EcN: Escherichia coli Nissle 1917 EcN-MPER: recombinant EcN containing mper sequence in the ompF loci ELISA: Enzyme-linked immunosorbent assay ...
Exp Hematol 2003; 31: 109–117. Article PubMed Google Scholar Molendijk WJ, van Oudenaren A, van Dijk H, Daha MR, Benner R . Complement split product C5a mediates the lipopolysaccharide-induced mobilization of CFU-s and haemopoietic progenitor cells, but not the mobilization induced by ...
copri, the cultured bacterial suspension was centrifuged at 4000 rpm for 5 min, and the precipitate was washed with sterile PBS, centrifuged and resuspended in PBS (3 × 109 CFU/mL). Th2 cells were inoculated in 96-well plates and exposed to the inactivated P. copri (0, 3.2 ×...
The prebiotic component was given at a dose of 2 mg and bacteria at a quantity of 105 cfu per egg. For further investigations only those roosters were qualified which had hatched from eggs previously in ovo injected into air chamber eggs (total 2400). The roosters were reared on a ...
(Meijerink2021). Shibat El-hamd and Ahmed (2016) observed that the administration of a commercial probiotic preparation in dose 108CFU/mL (combination of several probiotic strains includingL. plantarum,L. acidophilus,Saccharomyces cerevisiae) improved the immune response in chickens challenged with...
Antimicrobian peptide HBD-1 was measured in the medium from comparments delimited by the amnion (AMN) and the choriodecidua (CHD) under basal conditions and after the three different modalities of infection with 1 × 106 CFU of Candida albicans. Boxes represent median concentration of HBD...
At different incubation times the number of viable bacteria in treated and non-treated control cultures was determined. For each strain, the susceptibility was expressed as percentage of survival, calculated as the ratio between the CFU recovered from cultures exposed to stress over those obtained in...
a–dConventional wild-type C57BL/6J mice were colonized with 109CFU ofD. newyorkensis(Dub),A. muciniphila(Akk),E. faecalis(EF) or vehicle (Veh) twice with 2-day break in between, then treated with DSS as previously described (n = 5). At D7 post-DSS administration, colon sampl...