BAL was performed by inflation of the lung three times with 0.4 ml ice-cold PBS via an intratracheal cannula. Volume of 100 ml were used for CFU analysis, the remaining BAL fluid was centrifuged at 1,200 r.p.m. for 5 min and the supernatant stored at À 80 1C. Lung tissue was ...
All the assays were performed at least three times. To compare the expression of RBG1, WRKY45, and PR2 between KO and RBG1res-NIL after inoculation with B. glumae, both seed types were soaked in a bacterial inoculum suspension whose concentration was adjusted to 108 CFU/ml with ...
We added 2.5% L.P dy-1 (4 × 108 cfu/mL) to the barley flour with seven-times deionized water to ferment at 30 ℃ for 24 h. After centrifugation (8, 000 ×g for 20 min), the supernatant of the fermented liquid was dried with vacuum freeze drier (Marin Christ, Germany) and ...
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monocytogenes whole cells could be directly assayed within a linear range of 5.4×103–108 cfu/mL and a visual limit of detection of 5.4×103 cfu/mL. Fe3O4 NPC-based method was more sensitive than the Fe3O4 NP-based method. All these attractive characteristics of highly sensitivity, ...
Compared to unfermented barley, after 24 h of incubation with Lactobacillus plantarum dy-1 (initial cell density 4 × 108 cfu/g) at 30 °C, the protein content in LFBE showed a significant increase to 34.94% ± 1.51%, and the sugar content markedly decreased to 34.35% ± 2.59%. The ...
The sterilized seeds were then inoculated by applying 1 mL 2 × 106 CFU/mL conidial suspension in a 90 mm sterile petri plate and incubated at 30 ℃ for 7 days in dark. The inoculated seeds were dried at 110 ℃ for 4 h and ground to power. For each sample, 10 g of peanut powder...
to an optical density corresponding to a 0.5 McFarland standard (approximately 1.5 × 108 CFU/mL) and used to inoculate 96-well microtitre plates containing cation-adjusted Mueller-Hinton medium (Becton Dickinson, USA) at colistin concentrations from 0.25 to 128 μg /mL. EDTA...
coli strain HB101 (108 CFU/ml) was added. At 3, 6, and 12 h, 50 ml of basolateral supernatants were collected, diluted, plated on LB agar, and incubated for 24 h, followed www.laboratoryinvestigation.org | Laboratory Investigation | Volume 94 July 2014 779 HIV-1 impairs HRPE barrier ...
To induce transgenic U2AF1(WT) or U2AF1(S34F) expression, doxycycline hyclate (75ng/mL, Millipore Sigma) in water was added to the media before cell seeding. CFU-Pre-B colonies were counted on days 7–8. Nanostring assay and analysis Samples for NanoString were generated similar to ...