Also, we confirmed the limit of detection of this assay at 6.8 CFU/mL according to 3σ criterion. Our assay shows good sensitivity to detect bacteria range from 52.5 to 5.25 × 104 CFU/mL. This assay’s reliability was also confirmed by detecting artificially contaminated pork samples. Thus,...
Sample 10-month-old leaves were collected, cut into 2 mm × 4 mm pieces and immediately placed into a 1.5 mL centrifuge tube containing 2.5% glutaraldehyde–4% paraformaldehyde fixing solution37. The tissues were vacuumed to bring them into full contact with the fixing solution and then...
A.3.3成果计算:菌落数(CFU)/件=2个平皿菌落数平均值×10。第119页A.4合格原则A.4.1消毒合格原则:细菌总数≤20cfu/件。A.4.2灭菌合格原则:未检出细菌(无菌检查合格)。A.5致病菌检测A.5.1应在可疑经软式内镜诊断操作导致感染时进行检测。A.5.2检测办法:在进行细菌总数检测旳同步,取混匀旳待检样品0.2ml,分别...
niger, use sterile saline TS containing 0.05% of polysorbate 80, and add sufficient sterile saline TS to obtain a count of about 1 × 108 cfu per mL. Alternatively, the stock culture organisms ma y be grown in a suitable liquid medium (i.e., Soybean–Casein Digest Broth or Sabouraud ...
Tumor-derived factors are thought to regulate thrombocytosis and erythrocytopenia in individuals with cancer; however, such factors have not yet been identified. Here we show that tumor cell-released kynurenine (Kyn) biases megakaryocytic–erythroid prog
that yielded a detection limit of 1 × 105CFU/mL VP. Whereas a larger diameter greater than 1.0 μm and a broad size distribution obviously impede the fluid dynamic behaviors during the chromatography on the test strip surface, causing a false-positive result. Our study shows that physiochemical...
We added 2.5% L.P dy-1 (4 × 108 cfu/mL) to the barley flour with seven-times deionized water to ferment at 30 ℃ for 24 h. After centrifugation (8, 000 ×g for 20 min), the supernatant of the fermented liquid was dried with vacuum freeze drier (Marin Christ, Germany) and ...
Caco-2 cells were incubated with 1.5 ml of DMEM without FBS containing either 1 × 107 CFU/ml LGG wild-type or msp1 mutant CMPG10200, 100 ng/ml wild-type, recombinant or TFMS-treated Msp1. DMEM without FBS was used as a negative control. After 16 h incubation at 37°C, cell ...
The DrNLRP3 inflammasome occurrence is optimized at 6 hpf embryos or 72 hpf larvae with Edwardsiella tarda immersion infection (108 CFU/ml) for 1–4 h as determined by significantly increased DrCaspase-A/-B activity at those times (Fig. 9, A and B). DrNLRP3 overexpression alone in ...
All the assays were performed at least three times. To compare the expression of RBG1, WRKY45, and PR2 between KO and RBG1res-NIL after inoculation with B. glumae, both seed types were soaked in a bacterial inoculum suspension whose concentration was adjusted to 108 CFU/ml with ...