BAL was performed by inflation of the lung three times with 0.4 ml ice-cold PBS via an intratracheal cannula. Volume of 100 ml were used for CFU analysis, the remaining BAL fluid was centrifuged at 1,200 r.p.m. for 5 min and the supernatant stored at À 80 1C. Lung tissue was ...
All the assays were performed at least three times. To compare the expression of RBG1, WRKY45, and PR2 between KO and RBG1res-NIL after inoculation with B. glumae, both seed types were soaked in a bacterial inoculum suspension whose concentration was adjusted to 108 CFU/ml with ...
We added 2.5% L.P dy-1 (4 × 108 cfu/mL) to the barley flour with seven-times deionized water to ferment at 30 ℃ for 24 h. After centrifugation (8, 000 ×g for 20 min), the supernatant of the fermented liquid was dried with vacuum freeze drier (Marin Christ, Germany) and ...
1-3 Years: 1/2 Teaspoon (2.5 mL) 4-13 Years: 1 Tablespoon (15 mL) 14 Years+: 2 Tablespoons (30 mL) Instructions Suggested Use: Shake gently before use. Take once daily following the dosing instructions listed below, or dos...
monocytogenes whole cells could be directly assayed within a linear range of 5.4×103–108 cfu/mL and a visual limit of detection of 5.4×103 cfu/mL. Fe3O4 NPC-based method was more sensitive than the Fe3O4 NP-based method. All these attractive characteristics of highly sensitivity, ...
The sterilized seeds were then inoculated by applying 1 mL 2 × 106 CFU/mL conidial suspension in a 90 mm sterile petri plate and incubated at 30 ℃ for 7 days in dark. The inoculated seeds were dried at 110 ℃ for 4 h and ground to power. For each sample, 10 g of peanut powder...
Compared to unfermented barley, after 24 h of incubation with Lactobacillus plantarum dy-1 (initial cell density 4 × 108 cfu/g) at 30 °C, the protein content in LFBE showed a significant increase to 34.94% ± 1.51%, and the sugar content markedly decreased to 34.35% ± 2.59%. The ...
to an optical density corresponding to a 0.5 McFarland standard (approximately 1.5 × 108 CFU/mL) and used to inoculate 96-well microtitre plates containing cation-adjusted Mueller-Hinton medium (Becton Dickinson, USA) at colistin concentrations from 0.25 to 128 μg /mL. EDTA...
pylori SS1 (1 × 107 CFU/ml) for 14 days during each cycle. Three cycles of treatment were carried out to establish GC mice model. After 180 days of treatment, mice were scarified for subsequent analysis. The tumor size per stomach were counted (n = 10 per group). Murine ...
However, at comparable inoculum, OG1RF (4 × 108 CFU/ml) showed more rapid mortality versus V583 (5 × 108 CFU/ml) in the first 48 hours (P = 0.0034; Additional data file 3). In a urinary tract infection model administering mixed equal inocula of V583 and OG1RF, OG1RF ...