Sample 10-month-old leaves were collected, cut into 2 mm × 4 mm pieces and immediately placed into a 1.5 mL centrifuge tube containing 2.5% glutaraldehyde–4% paraformaldehyde fixing solution37. The tissues were vacuumed to bring them into full contact with the fixing solution and then...
cereus RCS3 in the concentration of 1 × 1010 CFU/mL and 1 × 1012 CFU/mL, respectively for 70 days before challenge intraperitoneally with 200 μl of 1 × 109 copies/g V. harveyi. Show abstract Impact of probiotics, prebiotics, and synbiotics on digestive enzymes, oxidative stress, and ...
heating at approximately 37 °C. Finally, using a cell phone camera, we detect label-free drug-resistant bacteria as low as 104cfu/mL, which is higher than clinically required threshold (>105cfu/mL) by enhancing 1000 times of the limit of detection (LOD) of colorimetric nitrocefin assay. ...
The number of Salmonella was 2 × 109 CFU per ml for the CL-55-treated cultures and control. We did not find a significant difference in the rate of bacteria growth between the CL-55-treated or control cultures and demonstrated that CL-55 did not affect the growth of Salmonella in vitro...
In a study of 66 elderly people, a probi- otic group treated daily for 6 months with a fermented flavored oat drink containing 109 cfu/mL Bifidobacte- rium longum 46 (DSM 14,583) and B. longum 2 C (DSM 14,579) also showed higher and more diverse levels of bifidobacteria in ...
Caco-2 cells were incubated with 1.5 ml of DMEM without FBS containing either 1 × 107 CFU/ml LGG wild-type or msp1 mutant CMPG10200, 100 ng/ml wild-type, recombinant or TFMS-treated Msp1. DMEM without FBS was used as a negative control. After 16 h incubation at 37°C, cell ...
CFU: colony forming units. (B) Ca9-22 cells were treated with 10 ng/ml of TNF-α for 3 h and were then incubated with P. gingivalis ATCC 33277 for 1 h. P.gingivalis was stained using antiserum for P. gingivalis whole cells. Then localization of P. gingivalis in the cells was ...
Two- fold dilutions of test bacteria in the range 107-109 CFU were used as the inocula for LD50 determination using 6-9 mice per inoculum group. Inocula CFU geometric mean values were obtained and used for LD50 calculation by the method of Reed and Muench [69]. UTI model for ...
For colony formation, the collected cells were mixed with 1 ml or 2 ml of MethoCult H4034 Optimum methylcellulose medium (STEMCELL Technologies, 04034) and plated onto one or two 35-mm Petri dishes. Individual colonies were phenotyped after 13 d as a colony-forming unit (CFU)-GM,...
For viral transductions, cells at 60% confluency were incubated with retrovirus (viral titers of 108 to 109 CFU/ml) or adenovirus (MOI of 1 : 100) for 90 min at 37 °C with mild agitation at 20-min intervals, followed by the addition of culture media supplemented with 10 μg/ml ...