The detection was 5×101∼5×106 CFU/mL. Compared with the previously reported colorimetric sensors for MR-1, the sensitivity was increased by 99.54 % to 23 CFU/mL. This study uniquely integrates optical sensors and microfluidic technology for rapid ERB detection with enhanced portability. ...
第109页5.3内镜清洗消毒机工作流程5.3.1使用内镜清洗消毒机时应遵循产品阐明操作使用。5.3.2在使用内镜清洗消毒机进行清洗消毒之前,应先遵循5.2.1、5.2.3旳规定对内镜进行预解决和手工清洗。5.3.3如果所用内镜清洗消毒机无测漏功能,应先遵循5.2.2旳规定进行手工测漏。5.3.4将内镜置入内镜清洗消毒机内,并用专用旳...
1.2.3 建立幽门螺杆菌感染AGS细胞模型:收集对数生长期的AGS细胞接种于6孔培养板中,按1 ×106个细胞/孔,每孔加1ml培养液。用PH7. 2的PBS 收集平板上的幽门螺杆菌,稀释菌液浓度至1 ×109CFU /ml。按照25:1、50:1、100:1、200:1的感染复数 (MOI,细菌数: 细胞数)加入幽门螺杆菌菌液到AGS细胞中,混合培...
For the inactivation of theP. copri, the cultured bacterial suspension was centrifuged at 4000 rpm for 5 min, and the precipitate was washed with sterile PBS, centrifuged and resuspended in PBS (3 × 109CFU/mL). Th2 cells were inoculated in 96-well plates and exposed to the inactivated...
The recipient 10-week-old male C57BL/6 mice were gavaged with 109 CFU of K.pn (W14 or TH1) or E.coli in 200 μL culture medium once every two days for 4 weeks. K.pn EVs (50 ng/mouse) by tail vein injection every 2 days for 4 weeks, then blood pressure was measured using ...
plantarum NJAU-01 at 107 CFU/g and 109 CFU/g significantly lowered the protein carbonyl content and protein surface hydrophobicity compared with the control (P < 0.05). The total sulfhydryl contents in L. plantarum NJAU-01 groups were significantly higher than that of the group without...
However, utilizing refrigerated storage, cell counts exceeded 7 log cfu/g for both free and immobilized cells, reaching populations greater than 109 cfu per share during the 10-day period, with no detectable spoilage. An investigation was conducted into the potential resistance of novel, fortified ...
For the probiotic group, a 0.2 mL solution containing 10 to the power of 8 colony-forming units (CFU) of LGG was administered orally each day until the animals were sacrificed. For radiation therapy (RT), a single 14 Gy dose was directed at the abdominopelvic area using a 6 mega-...
We show that the limit of quantitation is 1.9×105 CFU/mL with this simple device, which is more than 10,000-times lower than is achieved with electrochemical impedance spectroscopy (EIS) and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF) on the same ...
Integrated with the enrichment of the Van-PEG-PLL-MNP nano-platform and electrochemiluminescence (ECL) detection, Listeria monocytogenes can be rapidly and accurately detected at levels as low as 10 cfu mL-1. The approach described herein holds great potential for realizing rapid and sensitive ...