NC Phillips,D Robinson,BG Winchester 摘要: Normal calf alpha-mannosidase activity exists in at least three forms separable by chromatography on DEAE-cellulose and by starch-gel electrophoresis. Two components, A and B, have optimum activity between pH3.75 and 4.75, but component C has an optimum...
Three types of alpha-D-mannosidase are present in human and murine lymphocytes. Their levels increased substantially when the cells were activated by T-cell mitogens, concanavalin A (Con A) and phytohaemagglutinin (PHA), and in the murine cells also by lipopolysaccharide (LPS), a B-cell mitog...
Golgi membranes from rat liver have been shown to contain an endo-alpha-D-mannosidase which can convert Glc1Man9GlcNAc to Man8GlcNAc with the release of Glc alpha 1---3Man (Lubas, W. A., and Spiro, R. G. (1987) J. Biol. Chem. 262, 3775-3781). We now report that this enzyme...
Mannosidase, alpha, class 1 (MAN1A1 (Golgi α-mannnosidase IA), Man1A2 (Golgi α-mannosidase IB), MAN1B1(ER α-mannosidase I), MAN1C1 (Golgi α-mannosidase... Kelley Moremen 被引量: 0发表: 2014年 alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN...
GlcNAc([3H]Man)5GlcNAc-mannosidase and p-nitrophenyl alpha-D-mannosidase activities copurify throughout the preparative procedure and show the same properties. In addition to mannosidase IA (Tabas, I., and Kornfeld, S. (1979) J. Biol. Chem. 254, 11655-11663), a second alpha-1,2-...
We have investigated the vacuolar delivery of alpha-mannosidase, a marker enzyme of the vacuolar membrane in the yeast Saccharomyces cerevisiae, and found that the enzyme has several unique characteristics in its biosynthesis and vacuolar delivery. alpha-Mannosidase has no typical signal sequence (Yoshih...
We have previously reported the substrate specificity of the cytosolic alpha-D-mannosidase purified from rat liver using Man9GlcNAc, i.e. Man alpha 1-2Man alpha 1-3(Man alpha 1-2Man alpha 1-6)Man alpha 1-6(Man alpha 1-2Man alpha 1-2Man alpha 1-3) Man beta 1-4G1cNAc, as subs...
We have investigated the vacuolar delivery of alpha-mannosidase, a marker enzyme of the vacuolar membrane in the yeast Saccharomyces cerevisiae, and found that the enzyme has several unique characteristics in its biosynthesis and vacuolar delivery. alpha-Mannosidase has no typical signal sequence (Yoshih...
We have identified a mannosidase in rat liver that releases alpha 1---2, alpha 1---3 and alpha 1---6 linked manose residues from oligosaccharide substrates, MannGlcNAc where n = 4-9. The end product of the reaction is Man alpha 1---3[Man alpha 1---6]Man beta 1---4GlcNAc. Th...
Lysosomal enzyme levels in human amniotic fluid cells in tissue culture 3 Beta-glucuronidase, N-acetyl-beta-D-glucosaminidase, alpha-mannosidase and acid p... J Butterworth,GR Sutherland,DM Broadhead,... - 《Clinical Genetics》 被引量: 0发表: 1974年 4-methylumbelliferyl -N-acetylglucosaminidase ...