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To produce chicken anti-rtCPA antibody, the birds to be vaccinated received 70 micrograms with complete Freund's adjuvant (Sigma-Aldrich, St Louis, MO, at a 50:50 ratio w:w; protein: adjuvant) on d 7 and 50 micrograms of rtCPA with incomplete Freund's adjuvant on d 14 and 21 ...
优势代理TRC、EP、CATO、TLC、USP、LGC、Sigma、ATCCThermoFisher、Dr.E 、Chromadex个一线品牌,接受定制,并提供完整质检报告与证书 成立时间: 2017-05-22 企业经济性质: 私营合伙企业 年营业额: 100万以下 经营模式: 贸易商,生产商, 员工人数: 11-50 注册资金: 100万人民币 公司评价 暂时还没有评价我...
oligomerized pre-pore complex is necessary for pore formation byClostridium septicumalphatoxin.”Molec. Microbiol.23: 551-558.). The Sup-T1 cell line used for all flow cytometry was obtained from Dr. William Hildebrand at OUHSC. All chemicals were obtained from Sigma Chemical Company and all ...
Mice were then injected subcutaneously with a total of 375 µg ovalbumin (Grade 5; Sigma-Aldrich) in PBS, mixed with 450 µg of Mycobacterium tuberculosis (Difco, BD Biosciences) in 100 µL complete Freund adjuvant (CFA) into both flanks and tail.36 Lymphatic vessels around immunization...
Human recombinant TNF-α, fMLF, luminol, isoluminol, cycloheximide, Pertussis toxin, SB 203580 and cytochalasin B were obtained from Sigma Chemical Co., St. Louis. Superoxide dismutase (SOD), catalase and horse radish peroxidase (HRP) were from Boehringer-Mannheim, Germany. Radiolabeled fMLF was...
Freshly prepared trypsin [2 ml of 25 mg/ml trypsin solution (Sigma, T1426-1G TLCK treated] in 1 mM HCl, 5 mM CaCl2) was added, the pH was maintained at 11.0, and the mixture was stirred overnight at 4 °C. The digestion was applied to a Source 15Q 16/10 column pre-equilibrated...
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| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) and β-Actin-Ab (AC-15, A5441) from Sigma (Steinheim, Germany), p-Cofilin1-Ab (hSer3-R, sc-12912-R), cofilin-Ab (E-8, sc-376476), goat anti-rabbit IgG-HRP (sc-2004) and goat anti-mouse IgG-HRP (sc-2005)...