| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
To produce chicken anti-rtCPA antibody, the birds to be vaccinated received 70 micrograms with complete Freund's adjuvant (Sigma-Aldrich, St Louis, MO, at a 50:50 ratio w:w; protein: adjuvant) on d 7 and 50 micrograms of rtCPA with incomplete Freund's adjuvant on d 14 and 21 ...
Background staining was blocked with 5% BSA (Sigma-Aldrich) and 4% goat serum (Sigma-Aldrich) in PBS. Immunostaining for LYVE-1, rabbit IgG; 1:1000 (Upstate) or monoclonal rat IgG; 1:1000 (R&D Systems), Prox1, rabbit IgG; 1:100 (AbCam), B220, CD4 and CD8 rat IgG; 1:250 (BD...
Freshly prepared trypsin [2 ml of 25 mg/ml trypsin solution (Sigma, T1426-1G TLCK treated] in 1 mM HCl, 5 mM CaCl2) was added, the pH was maintained at 11.0, and the mixture was stirred overnight at 4 °C. The digestion was applied to a Source 15Q 16/10 column pre-equilibrated...
Human recombinant TNF-α, fMLF, luminol, isoluminol, cycloheximide, Pertussis toxin, SB 203580 and cytochalasin B were obtained from Sigma Chemical Co., St. Louis. Superoxide dismutase (SOD), catalase and horse radish peroxidase (HRP) were from Boehringer-Mannheim, Germany. Radiolabeled fMLF was...
sigma aldrich | sigma | Life Technologies | Molecular Probes | gibco | invitrogen | thermo | Tonbo Biosciences | targetingsystems | sunrise sciene products | PEVIVA | MicroGEM | ModiQuest | LGC link | HILICON AB | Cellutron Life Technologies | Biomedical Research Service | bodycap medical | ...
Eight 2 ml tubes of IB suspension of full-length Cry6Aa were thawed and extracted in 80 ml final volume (1:5 dilution) of 10 mM CAPS, pH 11.0. The measured pH of 9.1 was then adjusted to 11.0 with NaOH. Freshly prepared trypsin [2 ml of 25 mg/ml trypsin solution (Sigma, T1426...
| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
oligomerized pre-pore complex is necessary for pore formation byClostridium septicumalphatoxin.”Molec. Microbiol.23: 551-558.). The Sup-T1 cell line used for all flow cytometry was obtained from Dr. William Hildebrand at OUHSC. All chemicals were obtained from Sigma Chemical Company and all ...