Yeast with a half-synthetic genomedoi:10.1038/s41587-023-02069-zAnne DoerrNature Biotechnology
Over the past decade, we have been working on the synthesis of world’s first synthetic yeast genome (Sc2.0), with six chromosomes having been successfully constructed [16,17,18,19,20,21,22,23]. One unique feature of the synthetic yeast genome is the genome-wide incorporation of loxPsym ...
To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that in 20–40% of unperturbed yeast ce
pythonbiologysystems-biologyyeastgenome-scale-modelsyeast-metabolomesaccharomyces-cerevisiaegenome-scale-metabolic-modelstandard-gem UpdatedOct 13, 2024 Jupyter Notebook Presentations and work done for University of Pittsburgh course, Synthetic Biology.
really shows our excellence in engineering biology at the chromosome level, and it would not be possible without the great collaborations between ourSc2.0international teams. I look forward to continuing working with this amazing team to complete the entire synthetic yeast genome in the years to ...
As these elements were currently linked with a UASCIT upstream region, we removed this region and assessed the strength of the core element itself and found that indeed these core elements allowed for slight, but detectable, transcription (Fig. 2a). Figure 2: UAS activation of synthetic core ...
This study applied a targeted metabolomics approach to evaluate metabolic engineering strategies to increase the availability of intracellular L-tyrosine in the yeast Saccharomyces cerevisiae CEN.PK. Our engineering strategies combined localized pathway engineering with global engineering of central metabolism, ...
The close correlation between the Tau pathology and the disease progression makes protein Tau a good biomarker for diagnosis. The protein is released from neurons into cerebrospinal fluid (CSF), and, indeed, several studies established that AD patients display CSF changes with elevated levels of ...
Using a replica based screening, twenty thousand yeast transformants were screened to identify transformants expressing heterologous gene sequences from J. curcas with enhanced ability to tolerate stress. From the screen we obtained 32 full length genes from J. curcas [GenBank accession numbers FJ...
Overall correlations are excellent, indicating that the Spo11 oligo map provides a faithful quantitative picture of DSB distributions genome-wide. The deviation from nonlinearity for microarray probes with lower values on both axes suggests that Spo11 oligos provide better dynamic range than the ...