A core theme in synthetic biology, "understanding by creating," inspired the effort to generate the first synthetic cell, JCVI-Syn1.0 (1). The project Sc2.0 is elevating this concept by attempting to create a synthetic version of a more evolved organism, Saccharomyces cerevisiae, a eukaryotic ...
Design of a synthetic yeast genome We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromoso... SM Richardson,LA ...
We have clearly resolved four chromosomal bands from fourPichiapastoris(Komagataellapastoris) strains by using contour-clamped homogeneous electric field gel electrophoresis. The size of theP.pastorischromosomal bands ranged from 1.7 Mb to 3....
One of the ambitious projects currently underway is Sc2.0: the design and synthesis of a complete eukaroyotic genome - Saccharomyces cerevisiae. This collection highlights the experimental work published in Nature Communications on redesigning the S. cerevisiae genome along with commentary from the ...
Genome Res. 24, 1698–1706 (2014). CAS PubMed PubMed Central Google Scholar Sharon, E. et al. Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters. Nat. Biotechnol. 30, 521–530 (2012). CAS PubMed PubMed Central Google Scholar ...
The rapid development of single-cell sequencing technologies makes it possible to explore cell heterogeneity of genome, epigenome, transcriptome, and cell interaction/communication in the context of a specific environment in a tissue. However, the accurate detection of individual cells within complex ...
PRIMED are accurate and comprehensive and are provided as downloadable Excel files, removing the need for future primer design, especially for large-scale functional analyses. Furthermore, the open-source GRS can be used broadly to retrieve genome information from custom or other annotated genomes, ...
genome sequencing of 16 diverse species of biotechnologically important yeast species by a Joint Genome Institute-led effort [15]; and the genome sequencing of 220 species (including 24 from the RIKEN Institute in Japan) led by the Y1000+ Projecti, which placed emphasis on sequencing at least ...
The genome-wide profile of the COS-5.44 sensitive deletion strains was examined using Gene Set Enrichment Analysis GSEA, [29] to identify enriched biological processes. GSEA has been considered advantageous for quantitative genome-wide profiles because it can take into account the entire profile, expl...
Genome variability generates phenotypic heterogeneity and is of relevance for adaptation to environmental change, but the extent of such variability in natural populations is still poorly understood. For example, selected Saccharomyces cerevisiae strains