Comments by Francis Collins, director of the National Center for Human Genome Research at the National Institutes of Health; Details on the project, a collaborative effort of 92 laboratories in several countries; How the sequencing accomplishment opens a new door in genetic studies; The human ...
transcription, the maintenance of genome structure and chromosome segregation in mitosis and meiosis1. Although the specific mechanisms by which this is accomplished vary considerably across the family, key aspects are shared: including single or double-strand DNA cleavage to ...
The complete genome of a laboratory strain was deciphered in 1997, indicating that it possesses about 5800 protein-coding genes, located on 16 chromosomes. Furthermore, its protenome (full set of proteins) is likely to be defined in the near future. In addition, recent developments in DNA ...
Double-strand breaks on YACs during yeast meiosis may reflect meiotic recombination in the human genome. Nat Genet. 1996 Aug; 13 (4):481–484.Klein S, Zenvirth D, Sherman A, Ried K, Rappold G, Simchen G (1996) Double-strand breaks on YACs during yeast mei- osis may reflect ...
A method to introduce defined mutations into the yeast genome enables saturation mutagenesis of a gene and genome-scale introduction of genetic variants. Our understanding of how genotype controls phenotype is limited by the scale at which we can precise
Mamm. Genome, 7 (1996), pp. 563-574 View in ScopusGoogle Scholar [25] N. Cougot, S. Babajko, B. Seraphin Cytoplasmic foci are sites of mRNA decay in human cells J. Cell Biol., 165 (2004), pp. 31-40 View in ScopusGoogle Scholar [26] D. Ingelfinger, D.J. Arndt-Jovin, R...
moleculeson the surface of only one yeast cell[244,245]. EBY100 as an engineeredS. cerevisiaestrain can be utilized in Aga1-Aga2 yeast display system. In this approach, the Aga2pfusion proteinis encoded in an engineered plasmid whereas Aga1p is encoded in the yeast genome. Both are ...
Materials and methods Whole genome analyses suggest this trait evolved around the same time as fruiting plants some 100 Vineyard samples million years ago (Wolfe and Shields, 1997; Piskur et al., 2006). As well as harnessing this trait to make wine, bread and beer, humans have more ...
synthesize the first eukaryotic genome58,59. InSc2.0, LoxPsym sites are inserted across the genome after the ORF of each non-essential gene to enable massive genome shuffling and rapidly generate phenotypic diversity, a technology referred to as SCRaMbLE (Synthetic Chromosome Rearrangement and ...
The molecular basis of how temperature affects cell metabolism has been a long-standing question in biology, where the main obstacles are the lack of high-quality data and methods to associate temperature effects on the function of individual proteins as