We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an op...
M. et al. Design of a synthetic yeast genome. Science 355, 1040–1044 (2017). Article ADS CAS PubMed Google Scholar Dymond, J. S. et al. Synthetic chromosome arms function in yeast and generate phenotypic diversity by design. Nature 477, 471–U124 (2011). Article ADS CAS PubMed ...
Nyerges, A. et al. Synthetic genomes unveil the effects of synonymous recoding. Preprint at bioRxiv https://doi.org/10.1101/2024.06.16.599206 (2024). Article PubMed PubMed Central Google Scholar Richardson, S. M. et al. Design of a synthetic yeast genome. Science 355, 1040–1044 (2017)...
We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source fram...
Design of a synthetic yeast genome We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromoso... SM Richardson,LA ...
Discrete protein assemblies ranging from hundreds of kilodaltons to hundreds of megadaltons in size are a ubiquitous feature of biological systems and perform highly specialized functions1,2. Despite remarkable recent progress in accurately designing new
Designing promoters with desirable properties is essential in synthetic biology. Human experts are skilled at identifying strong explicit patterns in small samples, while deep learning models excel at detecting implicit weak patterns in large datasets. B
In synthetic yeast promoters, for instance, the absence of a UAS region in the promoter results primarily in low basal levels of gene expression [3]. However, despite years of extensive research, a straightforward model for enhancer function remains elusive. Consequently, while enhancers can be ...
The capability to design microbiomes with predictable functions would enable new technologies for applications in health, agriculture, and bioprocessing. Towards this goal, we develop a model-guided approach to design synthetic human gut microbiomes for
The large enveloped virus SARS-CoV-2 with a single-stranded RNA genome prone to rapid mutation with a positive sense of approximately 29.9 kb in length, is a member of the beta-coronavirus belonging to theCoronaviridaethat can cause infection and pneumonia in birds, mammals, and humans7,8. ...