Yamane H , Lee SJ , Kim BD , Tao R & Rose JK ( 2005 ) A coupled yeast signal sequence trap and transient plant expression strategy to identify genes encoding secreted proteins from peach pistils . J Exp Bot 56 : 2229 – 2238 ....
A TFP library was constructed from a genomic and truncated cDNA library by using the invertase-based signal sequence trap technique. The efficiency of the system was demonstrated using two rarely secreted proteins, human interleukin (hIL)-2 and hIL-32. Optimal TFPs for secretion of hIL-2 and ...
We discuss the advantages of using Saccharomyces cerevisiae for signal sequence trap technology. The yeast protein-translocation system appears to be less discriminating than that of higher eukaryotes – for example, a Theileria parva cysteine protease gene containing a recessed, nonclassical signal allows...
How can we build a model to clarify the relationship between the quantity, direction, and sequence of PREs and the strength of pheromone-induced promoters to design a promoter that meets specific requirements? How can we improve the maneuverability of cell fusion for the transfer of large genomes...
Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed,...
Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp. Mol. Biol. Cell 9, 301–309 (1998). Article CAS PubMed PubMed Central Google Scholar Albuquerque-Wendt, A., Hütte, H. J., Buettner, F. F. R., Routier, F. H. & Bakker, H. ...
This can result in a prominent neutral loss ion (MHnn+ − H3PO4) particularly in ion trap type mass spectrometers where it often constitutes the most abundant fragment ion. This hinders sequence annotation, and in many cases the assignment of phosphopeptides and phosphorylation sites is not ...
agonist-stimulated phosphorylation of GPCR C-terminal tails is an initiating signal that promotes the recruitment of factors necessary for their ubiquitin-mediated internalization and desensitization.17,18In addition,cell cycle progressionis a coordinating signal that is essential for ubiquitination and degrad...
Eukaryotic SRP is a multi-subunit ribonucleoprotein complex, which binds to translating ribosomes exposing nascent SRP recognition sequences comprising N-terminal signal sequences and signal anchor (SA) sequences. The latter initially serve as targeting signals and ultimately as TM domains that anchor ...
coli (Chakane et al., 2017) for use in yeast, we first codon-optimized it for yeast expression and then fused it to the yeast α-factor leader-sequence to target hemoglobin to the endoplasmic reticulum (ER) and the secretory pathway for extracellular secretion through the transport vesicles ...